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Mouse Monoclonal Caveolin-1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human, Rat samples. Cited in 36 publications. Immunogen corresponding to Cell preparation containing Cav1 protein.


Images

Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (AB17052), expandable thumbnail
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (AB17052), expandable thumbnail
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (AB17052), expandable thumbnail
  • Western blot - Anti-Caveolin-1 antibody [7C8] (AB17052), expandable thumbnail
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (AB17052), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing Cav1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P41350

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Predicted
Mouse
Predicted
Not recommended
Predicted
Predicted
Rat
Predicted
Not recommended
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
-
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human, Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

May act as a scaffolding protein within caveolar membranes (PubMed:11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed:19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed:25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).

Alternative names

CAV, CAV1, Caveolin-1

Recommended products

Mouse Monoclonal Caveolin-1 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human, Rat samples. Cited in 36 publications. Immunogen corresponding to Cell preparation containing Cav1 protein.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing Cav1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P41350
Clone number
7C8
Purification technique
Affinity purification Protein G
Specificity

The monoclonal antibody 7C8 recognizes caveolin-1α as well as caveolin-1β, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat.

Concentration
Loading...
Purification notes

0.2 µm filtered

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.

Biological function summary

Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.

Pathways

Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.

Associated diseases and disorders

Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.

Product promise

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6 product images

  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)

    ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)

    ab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.

  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052), expandable thumbnail

    Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)

    ab17052 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab17052 at 1/500 dilution and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 ?g/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 ?g/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052), expandable thumbnail

    Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)

    Lanes 1 - 4: Merged signal (red and green). Green - ab17052 observed at 21-24 kDa. Red - loading control, Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

    ab17052 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab17052 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052) at 1 µg/mL

    Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human CAV1 (Caveolin-1) knockout A-431 cell line (Human CAV1 (Caveolin-1) knockout A-431 cell line ab269583)

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 20 kDa

    Observed band size: 21-24 kDa

  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)

    Immunocytochemical analysis of Mouse Bend.3 cells, labeling Caveolin-1 with ab17052. Cells were paraformaldehyde fixed, permeabilized with PBS + 0.1% Triton (PBT), and blocked with 10% serum for 1 hour at 22°C. Staining with ab17052 (diluted 1/200) was for 16 hours at 4°C.

  • Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)

    All lanes: Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052) at 1/500 dilution

    All lanes: HUVEC whole cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 20 kDa, 49 kDa

    Exposure time: 10s

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