Rabbit Polyclonal Caveolin-1 antibody. Caveola marker. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 28 publications.
Images
Publications
- NPJ Regenerative medicine 7:632022Myogenic tissue nanotransfection improves muscle torque recovery following volumetric muscle loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew Clark,Subhadip Ghatak,Poornachander Reddy Guda,Mohamed S El Masry,Yi Xuan,Amy Y Sato,Teresita Bellido,Chandan K SenPubMed 36266362
- Development (Cambridge, England) 149:2022Mural Wnt/β-catenin signaling regulates Lama2 expression to promote neurovascular unit maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaptarshi Biswas,Sanjid Shahriar,Nicholas P Giangreco,Panos Arvanitis,Markus Winkler,Nicholas P Tatonetti,William J Brunken,Tyler Cutforth,Dritan AgalliuPubMed 36098369
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Not recommended | Expected | Tested |
| Mouse | Not recommended | Tested | Tested |
| Rat | Not recommended | Expected | Tested |
| Cow | Not recommended | Predicted | Predicted |
| Dog | Not recommended | Predicted | Predicted |
| Pig | Not recommended | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Dog, Pig, Recombinant full length protein - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
May act as a scaffolding protein within caveolar membranes (PubMed:11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed:19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed:25893292).
Alternative names
Caveolin-1, CAV, CAV1
Recommended products
Rabbit Polyclonal Caveolin-1 antibody. Caveola marker. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 28 publications.
Alternative names
Caveolin-1, CAV, CAV1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.
- Biological function summary
Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.
- Pathways
Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.
- Associated diseases and disorders
Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
Caveolin 1 - Caveolae Marker was immunoprecipitated using 0.5mg Mouse Heart whole tissue lysate, 5µg of Rabbit polyclonal to Caveolin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18199.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 20kDa: Caveolin 1 - Caveolae Marker.All lanes: Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
Predicted band size: 20 kDa
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
Lanes 1 - 4: Merged signal (red and green). Green - ab18199 observed at 21-24 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab18199 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab18199 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/mL
Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 2: CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 21-24 kDa
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
All lanes: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/mL
Lane 1: Mouse Heart Lysate at 10 µg
Lane 2: Human Heart Lysate at 10 µg
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
All lanes: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Heart (Mouse) Tissue Lysate at 10 µg
Lane 4: Heart (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
ab18199 recognizes the full length tagged recombinant Caveolin 1 protein (Recombinant Human Caveolin-1 protein ab114170) which has an expected molecular weight of 46 kDa.
All lanes: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/mL
All lanes: Western blot - Recombinant Human Caveolin-1 protein (Recombinant Human Caveolin-1 protein ab114170) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Exposure time: 1min
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199)
ab18199 recognizes the full length tagged recombinant Caveolin 1 protein (Recombinant Human Caveolin-1 protein ab114170) which has an expected molecular weight of 46 kDa.
All lanes: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/mL
All lanes: Western blot - Recombinant Human Caveolin-1 protein (Recombinant Human Caveolin-1 protein ab114170) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Exposure time: 1min
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