AB36152

Anti-Caveolin-1 antibody - Caveolae Marker

Name: Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) | Abcam
Brand: Abcam Limited
SKU: AB36152
Price: 400 USD
Availability: InStock
Rating: 4 (10 reviews)

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What is this?

(10 Reviews)

(12 Publications)

Goat Polyclonal Caveolin-1 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human CAV1 aa 1-50.

View Alternative Names

CAV, CAV1, Caveolin-1

5 Images

Supplier Data

Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling Caveolin-1 with ab36152 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor^® 488 secondary antibody at 2 μg/mL (green). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody at 2 μg/mL.

Supplier Data

Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling Caveolin-1 with ab36152 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor^® 488 secondary antibody at 2 μg/mL (green). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody at 2 μg/mL.

Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Flow Cytometry (Intracellular) analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caveolin-1 with ab36152 at 10 μg/mL (blue line). Cells permeabilized with 0.5% Triton. Primary incubation overnight followed by Alexa Fluor^® 488 secondary antibody at 1 μg/mL.

IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor^® 488 secondary antibody.

Supplier Data

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Lane 1:

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) at 0.003 µg/mL

Lane 2:

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) at 0.001 µg/mL

Lane 1:

A431 cell lysate at 35 µg

Lane 2:

A549 cell lysate at 35 µg

Predicted band size: 20 kDa

false

Supplier Data

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

All lanes:

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) at 0.003 µg/mL

All lanes:

Human Heart lysate at 35 µg

Predicted band size: 20 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human CAV1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q03135

Specificity

No cross-reactivity expected to Caveolin-2 and Caveolin-3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-0.01 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.

Biological function summary

Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.

Pathways

Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.

Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes (PubMed : 11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed : 19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed : 25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).

See full target information CAV1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40002894

2025

SNX19 Interacts with Caveolin-1 and Flotillin-1 to Regulate DR Endocytosis and Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bibhas Amatya,Jacob Q M Polzin,Van A M Villar,Jiang Yang,Prasad Konkalmatt,Xiaoyan Wang,Raisha C Cadme,Peng Xu,John J Gildea,Santiago Cuevas,Ines Armando,Robin A Felder,Pedro A Jose,Hewang Lee

Cell death & disease 15:771 PubMed39443481

2024

Enhanced mGluR intracellular activity causes psychiatric alterations in Niemann Pick type C disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Toledano-Zaragoza,Violeta Enriquez-Zarralanga,Sara Naya-Forcano,Víctor Briz,Rocío Alfaro-Ruíz,Miguel Parra-Martínez,Daniel N Mitroi,Rafael Luján,José A Esteban,María Dolores Ledesma

American journal of physiology. Renal physiology 322:F197-F207 PubMed35001664

2022

K7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Dieniffer Peixoto-Neves,Praghalathan Kanthakumar,Jeremiah M Afolabi,Hitesh Soni,Randal K Buddington,Adebowale Adebiyi

Nutrients 13: PubMed34836326

2021

Icariin Protects H9c2 Rat Cardiomyoblasts from Doxorubicin-Induced Cardiotoxicity: Role of Caveolin-1 Upregulation and Enhanced Autophagic Response.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Scicchitano,Cristina Carresi,Saverio Nucera,Stefano Ruga,Jessica Maiuolo,Roberta Macrì,Federica Scarano,Francesca Bosco,Rocco Mollace,Antonio Cardamone,Anna Rita Coppoletta,Lorenza Guarnieri,Maria Caterina Zito,Irene Bava,Luca Cariati,Marta Greco,Daniela Patrizia Foti,Ernesto Palma,Micaela Gliozzi,Vincenzo Musolino,Vincenzo Mollace

Brain research bulletin 174:153-160 PubMed34139316

2021

Efnb2 haploinsufficiency induces early gap junction plaque disassembly and endocytosis in the cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Defourny,Christophe Audouard,Alice Davy,Marc Thiry

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12785-12804 PubMed32744386

2020

Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrin Diem,Michael Fauler,Giorgio Fois,Andreas Hellmann,Natalie Winokurow,Stefan Schumacher,Christine Kranz,Manfred Frick

Scientific reports 9:7519 PubMed31101909

2019

Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuta Takagaki,Sen Shi,Makoto Katoh,Munehiro Kitada,Keizo Kanasaki,Daisuke Koya

Proceedings of the National Academy of Sciences of 115:E11120-E11127 PubMed30397118

2018

Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara L Lenis,Jane Hu,Sze Yin Ng,Zhichun Jiang,Shanta Sarfare,Marcia B Lloyd,Nicholas J Esposito,William Samuel,Cynthia Jaworski,Dean Bok,Silvia C Finnemann,Monte J Radeke,T Michael Redmond,Gabriel H Travis,Roxana A Radu

Scientific reports 8:14359 PubMed30254327

2018

Medium throughput breathing human primary cell alveolus-on-chip model.

Applications

Unspecified application

Species

Unspecified reactive species

Janick D Stucki,Nina Hobi,Artur Galimov,Andreas O Stucki,Nicole Schneider-Daum,Claus-Michael Lehr,Hanno Huwer,Manfred Frick,Manuela Funke-Chambour,Thomas Geiser,Olivier T Guenat

Cell calcium 53:187-94 PubMed23261316

2012

Calcium entry via TRPC1 channels activates chloride currents in human glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vishnu Anand Cuddapah,Kathryn L Turner,Harald Sontheimer

View all publications

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Product promise

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Anti-Caveolin-1 antibody - Caveolae Marker

Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (AB36152)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise