Goat Polyclonal Caveolin-1 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CAV1 aa 1-50.
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- American journal of physiology. Renal physiology 322:F197-F2072022K7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDieniffer Peixoto-Neves,Praghalathan Kanthakumar,Jeremiah M Afolabi,Hitesh Soni,Randal K Buddington,Adebowale AdebiyiPubMed 35001664
- Nutrients 13:2021Icariin Protects H9c2 Rat Cardiomyoblasts from Doxorubicin-Induced Cardiotoxicity: Role of Caveolin-1 Upregulation and Enhanced Autophagic Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam Scicchitano,Cristina Carresi,Saverio Nucera,Stefano Ruga,Jessica Maiuolo,Roberta Macrì,Federica Scarano,Francesca Bosco,Rocco Mollace,Antonio Cardamone,Anna Rita Coppoletta,Lorenza Guarnieri,Maria Caterina Zito,Irene Bava,Luca Cariati,Marta Greco,Daniela Patrizia Foti,Ernesto Palma,Micaela Gliozzi,Vincenzo Musolino,Vincenzo MollacePubMed 34836326
- Brain research bulletin 174:153-1602021Efnb2 haploinsufficiency induces early gap junction plaque disassembly and endocytosis in the cochlea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean Defourny,Christophe Audouard,Alice Davy,Marc ThiryPubMed 34139316
- Scientific reports 9:75192019Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuta Takagaki et. al.PubMed 31101909
- Proceedings of the National Academy of Sciences of 115:E11120-E111272018Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamara L Lenis et. al.PubMed 30397118
- Scientific reports 8:143592018Medium throughput breathing human primary cell alveolus-on-chip model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanick D Stucki,Nina Hobi,Artur Galimov,Andreas O Stucki,Nicole Schneider-Daum,Claus-Michael Lehr,Hanno Huwer,Manfred Frick,Manuela Funke-Chambour,Thomas Geiser,Olivier T GuenatPubMed 30254327
- Cell calcium 53:187-942012Calcium entry via TRPC1 channels activates chloride currents in human glioma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVishnu Anand Cuddapah,Kathryn L Turner,Harald SontheimerPubMed 23261316
- Journal of virology 85:334-472010The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus.Applications:WBReactive species:HumanCatherine L Hunt,Andrey A Kolokoltsov,Robert A Davey,Wendy MauryPubMed 21047970
- The Journal of neuroscience : the official journal of the Society for Neuroscience 28:8568-762008cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I.Applications:IHC-FoFrReactive species:MouseAchim Schmidtko,Wei Gao,Peter König,Sandra Heine,Roberto Motterlini,Peter Ruth,Jens Schlossmann,Doris Koesling,Ellen Niederberger,Irmgard Tegeder,Andreas Friebe,Gerd GeisslingerPubMed 18716216
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CAV1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q03135
Consider this alternative
Reactivity data
Select an application| ICC | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.001-0.01 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig | Dilution info - | Notes - |
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Target data
Function
May act as a scaffolding protein within caveolar membranes (PubMed:11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed:19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed:25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
Alternative names
CAV, CAV1, Caveolin-1
Recommended products
Goat Polyclonal Caveolin-1 antibody. Suitable for ICC, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CAV1 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CAV1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q03135
- Purification technique
- Affinity purification Immunogen
- Specificity
No cross-reactivity expected to Caveolin-2 and Caveolin-3.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.
- Biological function summary
Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.
- Pathways
Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.
- Associated diseases and disorders
Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling Caveolin-1 with ab36152 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 μg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 μg/mL.
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152)
Caveolin-1 Western blot staining of Human Heart lysate using goat Anti-Caveolin-1 antibody
All lanes: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) at 0.003 µg/mL
All lanes: Human Heart lysate at 35 µg
Predicted band size: 20 kDa
Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling Caveolin-1 with ab36152 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 μg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 μg/mL.
Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)
Flow Cytometry (Intracellular) analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caveolin-1 with ab36152 at 10 μg/mL (blue line). Cells permeabilized with 0.5% Triton. Primary incubation overnight followed by Alexa Fluor® 488 secondary antibody at 1 μg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152)
Caveolin-1 Western blot staining using goat Anti-Caveolin-1 antibody
Lane 1: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) at 0.003 µg/mL
Lane 2: Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab36152) at 0.001 µg/mL
Lane 1: A431 cell lysate at 35 µg
Lane 2: A549 cell lysate at 35 µg
Predicted band size: 20 kDa
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