Rabbit Recombinant Monoclonal Caveolin-1 antibody. N-terminal. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 12 publications.
Images
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img138214.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869)")
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunocytochemistry-immunofluorescence-img125898.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27717.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869)")
![Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunoprecipitation-img88864.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869)")
![Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--flow-cytometry-intracellular-img50446.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB192869)")
Publications
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- Journal of the European Academy of Dermatology and Venereology : JEADV 37:1920-19272023Interleukin-37 inhibits desmoglein-3 endocytosis and keratinocyte dissociation via upregulation of Caveolin-1 and inhibition of the STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunqin Liang,Fengxia Hu,Lidan Mao,Yun Qiu,Fanhe Jiang,Qian Wang,Kailibinuer Abulikemu,Yongzhen Hong,Xinyu Ge,Xiaojing KangPubMed 37262304
- Open life sciences 18:202205872023Shear stress leads to the dysfunction of endothelial cells through the Cav-1-mediated KLF2/eNOS/ERK signaling pathway under physiological conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihua Wang,Bingyue Wang,Lan Jia,Haibo Yu,Zhe Wang,Fang Wei,Aili JiangPubMed 37077343
- Molecules (Basel, Switzerland) 27:20225,2'-Dibromo-2,4',5'-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxia Yuan,Qianyi Hou,Xiue Feng,Yuanlin Zhang,Fan Yang,Rui Ge,Qingshan LiPubMed 35566232
- Translational vision science & technology 11:152022A Pilot Application of an iTRAQ-Based Proteomics Screen Estimates the Effects of Cigarette Smokers' Serum on RPE Cells With AMD High-Risk Alleles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBincui Cai,Zhe Zhang,Shuo Sun,Tingting Lin,Yifeng Ke,Zhiqing Li,Jin Yang,Xiaorong LiPubMed 35138344
- The FEBS journal 287:5478-54972020JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Liu,Jiaying Fan,Yan Yan,Xuening Dang,Ran Zhao,Yimei Xu,Zhide DingPubMed 32279424
- International journal of molecular sciences 21:2020miR-20b and miR-451a Are Involved in Gastric Carcinogenesis through the PI3K/AKT/mTOR Signaling Pathway: Data from Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Streleckiene et. al.PubMed 32013265
- Oncology letters 17:2867-28732019Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenli Chen et. al.PubMed 30854062
- Leukemia & lymphoma 60:1697-17082018Retinoic acid and 6-formylindolo(3,2-b)carbazole (FICZ) combination therapy reveals putative targets for enhancing response in non-APL AML.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodica P Bunaciu,Robert J MacDonald,Holly A Jensen,Feng Gao,Xin Wang,Lynn Johnson,Jeffrey D Varner,Andrew YenPubMed 30570341
- eLife 7:2018FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoledad A Camolotto et. al.PubMed 30475207
- Antioxidants & redox signaling 30:927-9442018Inducible Knockdown of Endothelial Protein Tyrosine Phosphatase-1B Promotes Neointima Formation in Obese Mice by Enhancing Endothelial Senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianne Jäger,Astrid Hubert,Rajinikanth Gogiraju,Magdalena L Bochenek,Thomas Münzel,Katrin SchäferPubMed 29390191
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/120 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/120 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
May act as a scaffolding protein within caveolar membranes (PubMed:11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed:19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed:25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
Alternative names
CAV, CAV1, Caveolin-1
Recommended products
Rabbit Recombinant Monoclonal Caveolin-1 antibody. N-terminal. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15554
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.
- Biological function summary
Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.
- Pathways
Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.
- Associated diseases and disorders
Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img138214.jpg "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Western blot staining using rabbit Anti-Caveolin-1 antibody
This data was developed using the same antibody clone in a different buffer formulation (ab192869).
Lanes 1 - 4: Merged signal (red and green). Green - ab192869 observed at 21-24 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab192869 was shown to react with Caveolin-1 in wild-type A-431 cells in western blot. Loss of signal was observed when CAV1 knockout cell line Human CAV1 (Caveolin-1) knockout A-431 cell line ab269583 (knockout cell lysate Human CAV1 (Caveolin-1) knockout A-431 cell lysate ab270706) was used. Wild-type A-431 and CAV1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab192869 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869) at 1/1000 dilution
Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 2: CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human CAV1 (Caveolin-1) knockout A-431 cell line (Human CAV1 (Caveolin-1) knockout A-431 cell line ab269583)
Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 21-24 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunocytochemistry-immunofluorescence-img125898.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Caveolin-1 antibody
ab192869 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192869 at 1/500 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 ?g/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 ?g/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27717.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-1 antibody
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunoprecipitation-img88864.jpg "Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Immunoprecipitation analysis of A431 cell lysate labeling Caveolin-1 using ab192869 at 1/30 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody.
All lanes: Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Predicted band size: 20 kDa
![Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--flow-cytometry-intracellular-img50446.jpg "Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Flow Cytometry (Intracellular) staining using rabbit Anti-Caveolin-1 antibody
Intracellular Intracellular Flow Cytometry overlay histogram showing wild-type HeLa (green line) and CAV1 knockout HeLa cells (Human CAV1 (Caveolin-1) knockout HeLa cell line ab255371) stained with ab192869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab192869) (1x106 in 100μl at 0.04 μg/ml) for 30 min at 22°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluorr® 488, pre-adsorbed) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used at 1/2000 for 30 min at 22°C.
Isotype control antibody was Rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line CAV1 knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img738.jpg "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Western blot staining using rabbit Anti-Caveolin-1 antibody
Lanes 1 - 4: Merged signal (red and green). Green - ab192869 observed at 20 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.ab192869 was shown to react with Caveolin-1 in wild-type HeLa. Loss of signal was observed when knockout cell line Human CAV1 (Caveolin-1) knockout HeLa cell line ab255371 (knockout cell lysate Human CAV1 (Caveolin-1) knockout HeLa cell lysate ab263806) was used. Wild-type and Caveolin-1 knockout samples were subjected to SDS-PAGE. ab192869 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869) at 1/10000 dilution
Lane 1: A431 cell lysate at 20 µg
Lane 2: A549 cell lysate at 20 µg
Lane 2: Western blot - Human CAV1 (Caveolin-1) knockout HeLa cell line (Human CAV1 (Caveolin-1) knockout HeLa cell line ab255371)
Lane 3: Wild-type HeLa cell lysate at 20 µg
Lane 4: CAV1 knockout HeLa cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 37 kDa
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img153184.jpg "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Western blot staining using rabbit Anti-Caveolin-1 antibody
All lanes: Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869) at 1/10000 dilution
Lane 1: PC-3 cell lysate at 10 µg
Lane 2: A549 cell lysate at 10 µg
Lane 3: A431 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30474.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-1 antibody
Immunohistochemical analysis of paraffin-embeddedMouse lung tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9560.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-1 antibody
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunocytochemistry-immunofluorescence-img20632.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Caveolin-1 antibody
Immunocytochemistry/Immunofluorescence analysis of A-673 cells labelling Caveolin-1 with ab192869 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Alexa Fluor® 594-conjugated anti-Tubulin [DM1A] at a dilution of 1/200. Nuclei counterstained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--flow-cytometry-intracellular-img114782.jpg "Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Flow Cytometry (Intracellular) staining using rabbit Anti-Caveolin-1 antibody
Intracellular Flow Cytometry analysis of NIH3T3 cells labeling Caveolin-1 using ab192869 at a 1/120 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody Blue. Rabbit monoclonal IgG was used as isotype control (Black).
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img442382.jpg "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)
Caveolin-1 Western blot staining using rabbit Anti-Caveolin-1 antibody
Caveolin-1 western blot using anti-Caveolin-1 antibody [EPR15554] - N-terminal ab192869. Publication image and figure legend from Streleckiene, G., Inciuraite, R., et al., 2020, Int J Mol Sci, PubMed 32013265.
ab192869 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab192869 please see the product overview.
CAV1 and TSC1 proteins level changes after exogenous up-regulation of miR-451a expression. CAV1 (A) and TSC1 (B) protein expression comparison 48 h and 72 h after transfection in AGS and MKN28 cell cultures transfected with miR-451a and miR-control. Significant CAV1 and TSC1 proteins’ level decrease was determined 72 h after transfection in AGS cell culture. Representative pictures of CAV1 and TSC1 proteins signals detected by Western Blot presented at the bottom of a figure (* p < 0.05). Data from three independent experiments.
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img738.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img153184.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30474.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9560.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--immunocytochemistry-immunofluorescence-img20632.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
![Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--flow-cytometry-intracellular-img114782.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
![Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869), expandable thumbnail](https://content.abcam.com/products/images/caveolin-1-antibody-epr15554-n-terminal-caveolae-marker-ab192869--western-blot-img442382.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (ab192869)")
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