Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells in the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227636 for 30 mins at room temperature.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human lung, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227636 for 30 mins at room temperature.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human colon tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human liver tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

Formalin-fixed, paraffin-embedded human lung tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (AB227636)

ab227636 was shown to react with CAV1 in wild-type U-87 MG cells in Western blot with loss of signal observed in CAV1 knockout cell line ab306811. Wild-type U-87 MG and CAV1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab227636 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Caveolin-1 antibody [SP43] - C-terminal - Caveolae Marker (ab227636) at 1/500 dilution

Lane 1:

Wild-type U-87 MG lysate at 20 µg

Lane 2:

CAV1 knock-out U-87 MG lysate at 20 µg

Lane 2:

Western blot - Human CAV1 knockout U-87 MG cell line (ab306811)

Observed band size: 20-22 kDa

false