AB288598

Anti-Caveolin 1 antibody [YCA-R15554-53-6 H3L2] - BSA and Azide free (Capture)

Recombinant
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Rabbit Recombinant Monoclonal Caveolin-1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CAV, CAV1, Caveolin-1

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Sandwich ELISA - Anti-Caveolin 1 antibody [YCA-R15554-53-6 H3L2] - BSA and Azide free (Capture) (AB288598)

sELISA

Lab

Sandwich ELISA - Anti-Caveolin 1 antibody [YCA-R15554-53-6 H3L2] - BSA and Azide free (Capture) (AB288598)

Sandwich ELISA of ab288634 with the capture antibody ab288598 dilution at 2 µg/mL and detector antibody ab288616 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

YCA-R15554-53-6 H3L2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab288598 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Caveolin-1. The recommended pair for sandwich ELISA is:
Capture: ab288598, Human Caveolin-1 Capture Antibody (unconjugated)
Detector: ab288616, Human Caveolin-1 Detector Antibody (unconjugated)
The reference range value is 0.2-12.5 ng/mL.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-1 also called CAV1 is an important component of caveolae structures in plasma membranes. Caveolin-1 is a structural protein with a molecular mass of about 22 kDa. It is highly expressed in adipocytes endothelial cells fibroblasts and muscle cells. The protein facilitates caveolae formation by binding cholesterol sphingolipids and signaling molecules which provides the characteristic flask-shaped invaginations in the cell membrane.

Biological function summary

Caveolin-1 influences several cellular processes including endocytosis signal transduction and lipid metabolism. It forms a complex with other proteins such as Cav2 which is essential for the functional caveolae assembly. Caveolin-1 interacts with numerous signaling molecules to modulate intracellular signaling pathways as it serves as a platform for various receptors and signaling complexes.

Pathways

Caveolin-1 participates actively in the MAPK/ERK and insulin signaling pathways. In the MAPK/ERK pathway caveolin-1 has interactions with proteins like Ras and Src kinases impacting cellular responses to growth factors. The presence of caveolin-1 in the insulin pathway helps regulate glucose uptake and metabolism through associations with various intracellular signals.

Caveolin-1 is connected to cancer and cardiovascular diseases. Its dysregulation affects angiogenesis and metastasis in cancers; higher levels have been observed in breast cancer cells. Additionally caveolin-1 is tied to atherosclerosis where it influences endothelial function. Interactions with proteins such as Akt and endothelial NO synthase are pivotal in these disease mechanisms further underlining caveolin-1's role in cellular regulation and disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes (PubMed : 11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed : 19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed : 25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).

See full target information CAV1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin 1 antibody [YCA-R15554-53-6 H3L2] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Caveolin 1 antibody [YCA-R15554-53-6 H3L2] - BSA and Azide free (Capture) (AB288598)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise