AB75876

Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker

RabMAb
Recombinant
KO Validated
Lab Essentials
20ul selling size
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(3 Publications)

Rabbit Recombinant Monoclonal Caveolin-1 phospho Y14 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 3 publications.

View Alternative Names

CAV, CAV1, Caveolin-1

3 Images

Lab

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (AB75876)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (ab75876) at 1/1000 dilution

Lane 1:

Untreated A673 (Human muscle Ewing's Sarcoma) whole cell lysates at 15 µg

Lane 2:

A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates at 15 µg

Lane 3:

A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (AB75876)

All lanes:

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (ab75876) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with pervanadate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

false

Unknown

Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (AB75876)

Dot blot analysis of Caveolin-1 (phospho Y14) phospho peptide (Lane 1), Caveolin-1 Non-phospho peptide (Lane 2) labelling Caveolin-1 (phospho Y14) with ab75876 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2288Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.

Biological function summary

Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.

Pathways

Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.

Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes (PubMed : 11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed : 19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed : 25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).

See full target information CAV1 phospho Y14

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:114 PubMed40328804

2025

Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Yang,Zhuoran Ma,Piaopiao Lian,Yi Wu,Ke Liu,Zhaoyuan Zhang,Zhicheng Tang,Yan Xu,Xuebing Cao

Experimental and therapeutic medicine 24:496 PubMed35837062

2022

Procyanidin B2 suppresses hyperglycemia-induced renal mesangial cell dysfunction by modulating CAV-1-dependent signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yin,Ke Wang,Xue Zhu,Guoyuan Lu,Donghua Jin,Junsi Qiu,Fanfan Zhou

FEBS open bio 11:3115-3125 PubMed34555268

2021

CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dali Zhao,Zhiqiang Yang,Chen Chen,Zhipeng Zhang,Yangsheng Yu,Zhituo Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (AB75876)

Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (AB75876)

Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - Caveolae Marker (AB75876)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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