AB97476

Anti-Caveolin-2 antibody

Name: Anti-Caveolin-2 antibody (ab97476) | Abcam
Brand: Abcam Limited
SKU: AB97476
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Caveolin-2 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CAV2 aa 1-50.

View Alternative Names

Caveolin-2, CAV2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody (AB97476)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody (AB97476)

Immunohistochemical analysis of Caveolin-2 in paraffin embedded FaDu xenograft, using ab97476 at a 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody (AB97476)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody (AB97476)

Immunofluorescence analysis of A431 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Caveolin 2 protein at membrane using ab97476 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Immunoprecipitation - Anti-Caveolin-2 antibody (AB97476)

Samples were separated by 15 % SDS PAGE. The immunoprecipitated caveolin 2 protein was detected by ab97476 diluted at 1/500.

All lanes:

Immunoprecipitation - Anti-Caveolin-2 antibody (ab97476) at 4 µg

Lane 1:

A549 whole cell extract at 30 µg

Lane 2:

A549 whole cell extract with Control IgG

Lane 3:

A549 whole cell extract with ab97476

Secondary

All lanes:

EasyBlot anti Rabbit IgG

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-Caveolin-2 antibody (AB97476)

12% SDS PAGE

All lanes:

Western blot - Anti-Caveolin-2 antibody (ab97476) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 18 kDa

false

Supplier Data

Western blot - Anti-Caveolin-2 antibody (AB97476)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Caveolin-2 antibody (ab97476) at 1/1000 dilution

All lanes:

C8D30 Whole cell extracts at 30 µg

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CAV2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P51636

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.

Biological function summary

Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.

Pathways

Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.

Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).

See full target information CAV2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Radiation oncology (London, England) 19:82 PubMed38926892

2024

Single-cell transcriptional analysis of irradiated skin reveals changes in fibroblast subpopulations and variability in caveolin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lionel E Kameni,Michelle Griffin,Charlotte E Berry,Siavash Shariatzadeh,Mauricio A Downer,Caleb Valencia,Alexander Z Fazilat,Rahim Nazerali,Arash Momeni,Michael Januszyk,Michael T Longaker,Derrick C Wan

Journal of clinical pathology 77:330-337 PubMed36854623

2024

Lipid droplets' functional protein caveolin-2 is associated with lipid metabolism-related molecule FABP5 and EMT marker E-cadherin in oral epithelial dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jie Chen,Yu-Ting Bai,Ji-Rong Xie,Gang Zhou

Journal of virology 89:11356-71 PubMed26339049

2015

HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3' Untranslated Region and Enhances Hepatitis C Virus Replication.

Applications

Species

Human

Shivaprasad Shwetha,Anuj Kumar,Ranajoy Mullick,Deeptha Vasudevan,Nilanjan Mukherjee,Saumitra Das

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody (AB97476)

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody (AB97476)

Immunoprecipitation - Anti-Caveolin-2 antibody (AB97476)

Western blot - Anti-Caveolin-2 antibody (AB97476)

Western blot - Anti-Caveolin-2 antibody (AB97476)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise