AB79397

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker

RabMAb
Recombinant
Lab Essentials
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(5 Publications)

Rabbit Recombinant Monoclonal Caveolin-2 antibody. Caveola marker. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Caveolin-2, CAV2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

ab79397 at 1/100 dilution staining Caveolin-2 in HeLa cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

ab79397 at 1/100 dilution staining Caveolin-2 in human uterus by Immunohistochemistry using paraffin-embedded tissue. Note staining of endothelial cells and smooth muscles.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caveolin-2 (red) with ab79397 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluorr^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

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Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

All lanes:

Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397) at 1/5000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Carrier free

Anti-Caveolin-2 antibody [EPR2220] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2220

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.

Biological function summary

Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.

Pathways

Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.

Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).

See full target information CAV2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 14:2001-2014 PubMed37497407

2023

CAVIN2/SDPR Functioned as a Tumor Suppressor in Lung Adenocarcinoma from Systematic Analysis of Caveolae-Related Genes and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Keyun Zhu,Baichuan Wang,Yingxi Li,Yue Yu,Zhaohui Chen,Haoran Yue,Qingxiang Meng,Dongchen Tian,Xiaofeng Liu,Weiyu Shen,Yao Tian

NAR cancer 4:zcac036 PubMed36381271

2022

Multiple-low-dose therapy: effective killing of high-grade serous ovarian cancer cells with ATR and CHK1 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Anya Golder,Louisa Nelson,Anthony Tighe,Bethany Barnes,Camilla Coulson-Gilmer,Robert D Morgan,Joanne C McGrail,Stephen S Taylor

Frontiers in oncology 11:703501 PubMed34513683

2021

Integrated Bioinformatic Analysis of the Expression and Prognosis of Caveolae-Related Genes in Human Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Tian,Xiaofeng Liu,Jing Hu,Huan Zhang,Baichuan Wang,Yingxi Li,Li Fu,Ran Su,Yue Yu

Scientific data 8:147 PubMed34050191

2021

High-content siRNA 3D co-cultures to identify myoepithelial cell-derived breast cancer suppressor proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hendrika M Duivenvoorden,Natasha K Brockwell,Cameron J Nowell,Kaylene J Simpson,Belinda S Parker

Cell and tissue research 374:121-136 PubMed29752569

2018

Widespread expression of perilipin 5 in normal human tissues and in diseases is restricted to distinct lipid droplet subpopulations.

Applications

Unspecified application

Species

Human

Merita Hashani,Hagen Roland Witzel,Lena Maria Pawella,Judith Lehmann-Koch,Jens Schumacher,Gunhild Mechtersheimer,Martina Schnölzer,Peter Schirmacher,Wilfried Roth,Beate Katharina Straub

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

Flow Cytometry (Intracellular) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (AB79397)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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