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AB133484

Anti-Caveolin-2 antibody [EPR5471]

2

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal Caveolin-2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

Caveolin-2, CAV2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (AB133484)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (AB133484)

ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Western blot - Anti-Caveolin-2 antibody [EPR5471] (AB133484)
  • WB

Unknown

Western blot - Anti-Caveolin-2 antibody [EPR5471] (AB133484)

All lanes:

Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

3T3-L1 cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

false

  • Carrier free

    Anti-Caveolin-2 antibody [EPR5471] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5471

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB32577

Anti-Caveolin-1 antibody [E249] - Caveolae Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (AB32577)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.
Biological function summary

Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.

Pathways

Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.

Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
See full target information CAV2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biological research 57:3 PubMed38217055

2024

MSC-derived exosomes protect auditory hair cells from neomycin-induced damage via autophagy regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Huijuan Kuang,Yiru Wang,Lili Bao,Wanxin Cao,Lu Yu,Meihao Qi,Renfeng Wang,Xiaoshan Yang,Qingyuan Ye,Feng Ding,Lili Ren,Siying Liu,Furong Ma,Shiyu Liu

Cell reports 32:108017 PubMed32814035

2020

Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Schmidt,Andreas Thywißen,Marie Goldmann,Cristina Cunha,Zoltán Cseresnyés,Hella Schmidt,Muhammad Rafiq,Silvia Galiani,Markus H Gräler,Georgios Chamilos,João F Lacerda,António Campos,Christian Eggeling,Marc Thilo Figge,Thorsten Heinekamp,Scott G Filler,Agostinho Carvalho,Axel A Brakhage

Cell death & disease 11:553 PubMed32694556

2020

CircRNA TADA2A relieves idiopathic pulmonary fibrosis by inhibiting proliferation and activation of fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Li,Ping Li,Guojun Zhang,Pan Qin,Da Zhang,Wei Zhao

EMBO molecular medicine 12:e09271 PubMed32187826

2020

Endothelial Notch signaling controls insulin transport in muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Sana S Hasan,Markus Jabs,Jacqueline Taylor,Lena Wiedmann,Thomas Leibing,Viola Nordström,Giuseppina Federico,Leticia P Roma,Christopher Carlein,Gretchen Wolff,Bilgen Ekim-Üstünel,Maik Brune,Iris Moll,Fabian Tetzlaff,Hermann-Josef Gröne,Thomas Fleming,Cyrill Géraud,Stephan Herzig,Peter P Nawroth,Andreas Fischer

PLoS pathogens 15:e1007938 PubMed31356638

2019

Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunling Wang,Henry Puerta-Guardo,Scott B Biering,Dustin R Glasner,Edwina B Tran,Mark Patana,Trent A Gomberg,Carmel Malvar,Nicholas T N Lo,Diego A Espinosa,Eva Harris

Cellular and molecular life sciences : CMLS 76:3005-3018 PubMed31006037

2019

NEAT1 regulates neuroglial cell mediating Aβ clearance via the epigenetic regulation of endocytosis-related genes expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqiang Wang,Yiwan Zhao,Naihan Xu,Shikuan Zhang,Songmao Wang,Yunhao Mao,Yuanchang Zhu,Bing Li,Yuyang Jiang,Ying Tan,Weidong Xie,Burton B Yang,Yaou Zhang
View all publications
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Product promise

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