Rabbit Recombinant Monoclonal Caveolin-2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (AB133484), expandable thumbnail](https://content.abcam.com/products/images/caveolin-2-antibody-epr5471-ab133484--immunocytochemistry-immunofluorescence-img114275.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (AB133484)")
![Western blot - Anti-Caveolin-2 antibody [EPR5471] (AB133484), expandable thumbnail](https://content.abcam.com/products/images/caveolin-2-antibody-epr5471-ab133484--western-blot-img102322.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-2 antibody [EPR5471] (AB133484)")
Publications
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- Cell reports 32:1080172020Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranziska Schmidt,Andreas Thywißen,Marie Goldmann,Cristina Cunha,Zoltán Cseresnyés,Hella Schmidt,Muhammad Rafiq,Silvia Galiani,Markus H Gräler,Georgios Chamilos,João F Lacerda,António Campos,Christian Eggeling,Marc Thilo Figge,Thorsten Heinekamp,Scott G Filler,Agostinho Carvalho,Axel A BrakhagePubMed 32814035
- Cell death & disease 11:5532020CircRNA TADA2A relieves idiopathic pulmonary fibrosis by inhibiting proliferation and activation of fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Li et. al.PubMed 32694556
- PLoS pathogens 15:e10079382019Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunling Wang et. al.PubMed 31356638
- Cellular and molecular life sciences : CMLS 76:3005-30182019NEAT1 regulates neuroglial cell mediating Aβ clearance via the epigenetic regulation of endocytosis-related genes expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqiang Wang et. al.PubMed 31006037
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Expected |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
Alternative names
Caveolin-2, CAV2
Recommended products
Rabbit Recombinant Monoclonal Caveolin-2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5471
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.
- Biological function summary
Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.
- Pathways
Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.
- Associated diseases and disorders
Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484), expandable thumbnail](https://content.abcam.com/products/images/caveolin-2-antibody-epr5471-ab133484--immunocytochemistry-immunofluorescence-img114275.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)")
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.
![Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484), expandable thumbnail](https://content.abcam.com/products/images/caveolin-2-antibody-epr5471-ab133484--western-blot-img102322.jpg "Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)")
Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
All lanes: Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution
Lane 1: HUVEC cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: 3T3-L1 cell lysate at 10 µg
Lane 4: U87-MG cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
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