AB3417

Anti-Caveolin-2 (phospho Y19) antibody

Name: Anti-Caveolin-2 (phospho Y19) antibody (ab3417) | Abcam
Brand: Abcam Limited
SKU: AB3417
Rating: 4 (4 reviews)

BOND RX™ Validated
What is this?

(4 Reviews)

(9 Publications)

Rabbit Polyclonal Caveolin-2 phospho Y19 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Mouse Cav2 phospho Y19 aa 1-50.

View Alternative Names

Caveolin-2

2 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 (phospho Y19) antibody (AB3417)

Immunocytochemistry/Immunofluorescence analysis of Phospho-Caveolin-2 pTyr19 (green) showing staining in the cytoplasm and nucleus of HUVEC cells treated with 100μM pervanadate (left) and untreated HUVEC cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3417 in 3% BSA-PBS at a dilution of 1 : 20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 (phospho Y19) antibody (AB3417)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 (phospho Y19) antibody (AB3417)

IHC image of ab3417 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3417, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Cav2 phospho Y19 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9WVC3

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.

Biological function summary

Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.

Pathways

Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.

Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).

See full target information Cav2 phospho Y19

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 532:535-540 PubMed32896381

2020

N-myristoylation regulates insulin-induced phosphorylation and ubiquitination of Caveolin-2 for insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hayeong Kwon,Moonjeong Choi,Yujin Ahn,Yunbae Pak

OncoTargets and therapy 11:6209-6216 PubMed30288056

2018

CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Liu,Zhi Shangli,Zhili Hu

Nucleic acids research 43:3114-27 PubMed25753664

2015

Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane.

Applications

WB, IM

Species

Rat, Rat

Kyuho Jeong,Hayeong Kwon,Jaewoong Lee,Donghwan Jang,Yunbae Pak

Biochimica et biophysica acta 1853:1022-34 PubMed25667086

2015

Fatty acylated caveolin-2 is a substrate of insulin receptor tyrosine kinase for insulin receptor substrate-1-directed signaling activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hayeong Kwon,Jaewoong Lee,Kyuho Jeong,Donghwan Jang,Yunbae Pak

FEBS letters 586:3317-23 PubMed22819829

2012

N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative effect of transforming growth factor beta in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Britain Abel,Cara Willoughby,Sungchan Jang,Laura Cooper,Leike Xie,Chi Vo-Ransdell,Grzegorz Sowa

Traffic (Copenhagen, Denmark) 13:1218-33 PubMed22607032

2012

Rab6-mediated retrograde transport regulates inner nuclear membrane targeting of caveolin-2 in response to insulin.

Applications

Unspecified application

Species

Unspecified reactive species

Kyuho Jeong,Hayeong Kwon,Jaewoong Lee,Donghwan Jang,Eun Mi Hwang,Jae-Yong Park,Yunbae Pak

Journal of cellular and molecular medicine 15:888-908 PubMed20455999

2010

A novel domain of caveolin-2 that controls nuclear targeting: regulation of insulin-specific ERK activation and nuclear translocation by caveolin-2.

Applications

Unspecified application

Species

Unspecified reactive species

Hayeong Kwon,Kyuho Jeong,Eun Mi Hwang,Jae-Yong Park,Yunbae Pak

Journal of cellular and molecular medicine 13:1549-64 PubMed19778377

2009

Identification of pY19-caveolin-2 as a positive regulator of insulin-stimulated actin cytoskeleton-dependent mitogenesis.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

Hayeong Kwon,Kyuho Jeong,Yunbae Pak

Biochimica et biophysica acta 1793:1325-33 PubMed19427337

2009

Caveolin-2 regulation of STAT3 transcriptional activation in response to insulin.

Applications

Species

Rat

Hayeong Kwon,Kyuho Jeong,Eun Mi Hwang,Jae-Yong Park,Seong-Geun Hong,Wan-Sung Choi,Yunbae Pak

View all publications

style

left-column

style

left-column

style

right-column