AB284759

Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] - BSA and Azide free

RabMAb
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Rabbit Monoclonal Caveolin-2 phospho Y27 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Caveolin-2, CAV2

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Western blot - Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] - BSA and Azide free (AB284759)

This data was developed using ab76405, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] - BSA and Azide free (ab284759) at 1/2000 dilution

Lane 1:

HeLa cell lysates untreated. at 10 µg

Lane 2:

HeLa cell lysates treated with pervanadate. at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Unconjugated

Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2221Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284759 is the carrier-free version of ab76405

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.

Biological function summary

Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.

Pathways

Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.

Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).

See full target information Caveolin-2 phospho Y27

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] - BSA and Azide free

Western blot - Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] - BSA and Azide free (AB284759)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise