AB87770

Anti-Caveolin-3 antibody - Caveolae Marker

Name: Anti-Caveolin-3 antibody - Caveolae Marker (ab87770) | Abcam
Brand: Abcam Limited
SKU: AB87770
Price: 545 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal Caveolin-3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Pig, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CAV3 aa 1-50.

View Alternative Names

Caveolin-3, M-caveolin, CAV3

3 Images

Unknown

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (AB87770)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab87770) at 0.3 µg/mL

All lanes:

human heart lysate in RIPA buffer at 35 µg

Predicted band size: 17 kDa

Observed band size: 23 kDa

true

Exposure time: 1hr

Supplier Data

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (AB87770)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab87770) at 0.3 µg/mL

Lane 1:

mouse heart tissue lysate at 35 µg

Lane 2:

rat heart tissue lysate at 35 µg

Predicted band size: 17 kDa

Observed band size: 20 kDa

false

Unknown

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (AB87770)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (ab87770) at 0.1 µg/mL

Lane 1:

pig skeletal muscle tissue lysate at 35 µg

Lane 2:

pig heart tissue lysate at 35 µg

Predicted band size: 17 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CAV3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P56539

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-3 also referred to as 'caveolin' 'caveolae' and 'CAV3' is a protein with an approximate mass of 17 kDa. It acts as a structural component of caveolae small invaginations in the plasma membrane. Caveolin-3 is expressed majorly in muscle cells including cardiac skeletal and smooth muscle tissues. As a distinguishing marker for caveolae it plays an essential role in the formation and stabilization of these structures.

Biological function summary

Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.

Pathways

Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.

Caveolin-3 mutations have been associated with a range of muscle-related disorders including limb-girdle muscular dystrophy and hypertrophic cardiomyopathy. These conditions often arise due to alterations in muscle cell signaling and integrity. Caveolin-3 also shares connections with proteins like dystrophin an important player in maintaining muscle cell structure and its dysfunction can exacerbate these muscular disorders. Understanding these interactions offers insights into potential therapeutic targets for managing related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed : 19262564).

See full target information CAV3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:3877-3895 PubMed35152831

2022

Uric acid, as a double-edged sword, affects the activity of epidermal growth factor (EGF) on human umbilical vein endothelial cells by regulating aging process.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Linru Zhao,Wufang Qi

Developmental cell 53:406-417.e5 PubMed32359406

2020

Intracellular Role for the Matrix-Modifying Enzyme Lox in Regulating Transcription Factor Subcellular Localization and Activity in Muscle Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ravit Gabay Yehezkely,Shelly Zaffryar-Eilot,Anna Kaganovsky,Nurit Fainshtain Malka,Rohtem Aviram,Ido Livneh,Peleg Hasson

Journal of orthopaedic research : official publica 37:1387-1397 PubMed30644571

2019

Enhancement of the chondrogenic differentiation of mesenchymal stem cells and cartilage repair by ghrelin.

Applications

Unspecified application

Species

Unspecified reactive species

Litong Fan,Jiaqing Chen,Yanmeng Tao,Boon Chin Heng,Jiakuo Yu,Zheng Yang,Zigang Ge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Caveolin-3 antibody - Caveolae Marker

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (AB87770)

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (AB87770)

Western blot - Anti-Caveolin-3 antibody - Caveolae Marker (AB87770)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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