Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free

• IHC-FoFr

Lab

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773)

Immunohistochemistry analysis of frozen mouse skeletal muscle tissue labeling Caveolin-3 with ab173575 at 1/1000 dilution. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Alexa Fluor^® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

Membranous staining on mouse skeletal muscle, while negative staining on white adipose tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173575).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773)

ab173575 at 1/50. C2C12 (Mouse myoblast cell line) cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an Alexa Fluor^® 594-conjugated mouse anti-alpha tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).

Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. No staining detected in undifferentiated C2C12 cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173575).

• WB

Lab

Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Mouse adipose tissue (PMID : 8663016).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173575).

All lanes:

Western blot - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-epr11082-caveolae-marker-ab173575'>ab173575</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse white adipose tissue lysate at 40 µg

Lane 3:

Mouse brown adipose tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Exposure time: 3min