Rabbit Recombinant Monoclonal Caveolin-3 antibody. Carrier free. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11082-bsa-and-azide-free-ab249773--immunohistochemistry-pfa-perfusion-fixed-frozen-sections-img44787.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773)")
![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11082-bsa-and-azide-free-ab249773--immunocytochemistry-immunofluorescence-img91784.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773)")
![Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11082-bsa-and-azide-free-ab249773--western-blot-img7719.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (AB249773)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Expected | Expected |
| Mouse | Not recommended | Expected | Tested | Expected | Tested |
| Rat | Not recommended | Predicted | Predicted | Expected | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed:19262564).
Alternative names
Caveolin-3, M-caveolin, CAV3
Recommended products
Rabbit Recombinant Monoclonal Caveolin-3 antibody. Carrier free. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11082
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249773 is the carrier-free version of Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Caveolin-3 also referred to as 'caveolin' 'caveolae' and 'CAV3' is a protein with an approximate mass of 17 kDa. It acts as a structural component of caveolae small invaginations in the plasma membrane. Caveolin-3 is expressed majorly in muscle cells including cardiac skeletal and smooth muscle tissues. As a distinguishing marker for caveolae it plays an essential role in the formation and stabilization of these structures.
- Biological function summary
Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.
- Pathways
Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.
- Associated diseases and disorders
Caveolin-3 mutations have been associated with a range of muscle-related disorders including limb-girdle muscular dystrophy and hypertrophic cardiomyopathy. These conditions often arise due to alterations in muscle cell signaling and integrity. Caveolin-3 also shares connections with proteins like dystrophin an important player in maintaining muscle cell structure and its dysfunction can exacerbate these muscular disorders. Understanding these interactions offers insights into potential therapeutic targets for managing related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11082-bsa-and-azide-free-ab249773--immunohistochemistry-pfa-perfusion-fixed-frozen-sections-img44787.jpg "Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)")
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
Immunohistochemistry analysis of frozen mouse skeletal muscle tissue labeling Caveolin-3 with Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575 at 1/1000 dilution. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, AlexaFluor® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Membranous staining on mouse skeletal muscle, while negative staining on white adipose tissue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575).![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11082-bsa-and-azide-free-ab249773--immunocytochemistry-immunofluorescence-img91784.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)")
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575 at 1/50. C2C12 (Mouse myoblast cell line) cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, an Alexa Fluor® 594-conjugated mouse anti-alpha tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).
Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. No staining detected in undifferentiated C2C12 cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575).
![Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11082-bsa-and-azide-free-ab249773--western-blot-img7719.jpg "Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)")
Western blot - Anti-Caveolin-3 antibody [EPR11082] - BSA and Azide free (ab249773)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Mouse adipose tissue (PMID: 8663016).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575).
All lanes: Western blot - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker ab173575) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse white adipose tissue lysate at 40 µg
Lane 3: Mouse brown adipose tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 3min
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