Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Caveolin-3 with ab173575 at 1/500 dilution (0.122 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on human cardiac muscle (PMID : 28356340) The section was incubated with ab173575 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Caveolin-3 with ab173575 at 1/500 dilution (0.122 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on mouse cardiac muscle (PMID : 28356340) The section was incubated with ab173575 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Caveolin-3 with ab173575 at 1/500 dilution (0.122 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on rat cardiac muscle (PMID : 28356340) The section was incubated with ab173575 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

Immunocytochemistry/Immunofluorescence analysis of C2C12 (mouse myoblast cell line) cells labelling Caveolin-3 with purified ab173575 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an Alexa Fluor^® 594-conjugated mouse anti-alpha tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).

Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. No staining detected in undifferentiated C2C12 cells.

• IHC-FoFr

Lab

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

Immunohistochemistry analysis of frozen mouse skeletal muscle tissue labeling Caveolin-3 with ab173575 at 1/1000 dilution. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Alexa Fluor^® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

Membranous staining on mouse skeletal muscle, while negative staining on white adipose tissue.

• WB

Lab

Western blot - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Mouse adipose tissue (PMID : 8663016).

All lanes:

Western blot - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (ab173575) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse white adipose tissue lysate at 40 µg

Lane 3:

Mouse brown adipose tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (AB173575)

All lanes:

Western blot - Anti-Caveolin-3 antibody [EPR11082] - Caveolae Marker (ab173575) at 1/1000 dilution

Lane 1:

Human Skeletal Muscle Lysate at 10 µg

Lane 2:

Fetal Heart Lysate at 10 µg

Predicted band size: 17 kDa

false