Rabbit Recombinant Monoclonal Caveolin-3 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--western-blot-img71193.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752)")
![Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunoprecipitation-img73893.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91308.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118140.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130488.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (AB171752)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Expected | Expected | Not recommended | Tested |
| Rat | Expected | Expected | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed:19262564).
Alternative names
Caveolin-3, M-caveolin, CAV3
Recommended products
Rabbit Recombinant Monoclonal Caveolin-3 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11083
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Caveolin-3 also referred to as 'caveolin' 'caveolae' and 'CAV3' is a protein with an approximate mass of 17 kDa. It acts as a structural component of caveolae small invaginations in the plasma membrane. Caveolin-3 is expressed majorly in muscle cells including cardiac skeletal and smooth muscle tissues. As a distinguishing marker for caveolae it plays an essential role in the formation and stabilization of these structures.
- Biological function summary
Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.
- Pathways
Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.
- Associated diseases and disorders
Caveolin-3 mutations have been associated with a range of muscle-related disorders including limb-girdle muscular dystrophy and hypertrophic cardiomyopathy. These conditions often arise due to alterations in muscle cell signaling and integrity. Caveolin-3 also shares connections with proteins like dystrophin an important player in maintaining muscle cell structure and its dysfunction can exacerbate these muscular disorders. Understanding these interactions offers insights into potential therapeutic targets for managing related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--western-blot-img71193.jpg "Western blot - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Western blot - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Caveolin-3 Western blot staining using rabbit Anti-Caveolin-3 antibody
All lanes: Western blot - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Mouse muscle lysate at 10 µg
Predicted band size: 17 kDa
![Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunoprecipitation-img73893.jpg "Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate labeling Caveolin 3 using ab171752 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Predicted band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91308.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Caveolin-3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-3 antibody
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human skeletal muscle (PMID: 11431690). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118140.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Caveolin-3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-3 antibody
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and weak cytoplasmic staining on human cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130488.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Caveolin-3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-3 antibody
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and weak cytoplasmic staining on rat cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100744.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Caveolin-3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-3 antibody
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and weak cytoplasmic staining on mouse skeletal muscle (PMID: 11431690). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10305.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)
Caveolin-3 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Caveolin-3 antibody
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Caveolin-3 with ab171752 at 1/4000 dilution (0.131 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and cytoplasmic staining on mouse cardiac muscle (PMID: 28356340). The section was incubated with ab171752 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100744.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752), expandable thumbnail](https://content.abcam.com/products/images/caveolin-3-antibody-epr11083-caveolae-marker-ab171752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10305.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR11083] - Caveolae Marker (ab171752)")
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