AB250637

Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Caveolin-3 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Caveolin-3, M-caveolin, CAV3

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative on Human hepatocellular carcinoma. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Human cardiac muscle was observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative on Human liver. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Caveolin-3 with ab182739 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Human skeletal muscle was observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Caveolin-3 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab182739 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab182739 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human fetal heart whole cell lysate 10ug (Input).

Lane 2 : ab182739 IP in Human fetal heart whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab182739 in Human fetal heart whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Caveolin-3 antibody [EPR18975] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-epr18975-caveolae-marker-ab182739'>ab182739</a>)

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Supplier Data

Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Expressed predominantly in muscle.

All lanes:

Western blot - Anti-Caveolin-3 antibody [EPR18975] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-epr18975-caveolae-marker-ab182739'>ab182739</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Human fetal lung lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

This data was developed using ab182739, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Expressed predominantly in muscle.

All lanes:

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Unconjugated

Anti-Caveolin-3 antibody [EPR18975] - Caveolae Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18975

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250637 is the carrier-free version of ab182739.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-3 also referred to as 'caveolin' 'caveolae' and 'CAV3' is a protein with an approximate mass of 17 kDa. It acts as a structural component of caveolae small invaginations in the plasma membrane. Caveolin-3 is expressed majorly in muscle cells including cardiac skeletal and smooth muscle tissues. As a distinguishing marker for caveolae it plays an essential role in the formation and stabilization of these structures.

Biological function summary

Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.

Pathways

Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.

Caveolin-3 mutations have been associated with a range of muscle-related disorders including limb-girdle muscular dystrophy and hypertrophic cardiomyopathy. These conditions often arise due to alterations in muscle cell signaling and integrity. Caveolin-3 also shares connections with proteins like dystrophin an important player in maintaining muscle cell structure and its dysfunction can exacerbate these muscular disorders. Understanding these interactions offers insights into potential therapeutic targets for managing related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed : 19262564).

See full target information CAV3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 33:451-462 PubMed35775321

2022

Notoginsenoside R1 alleviates spinal cord injury by inhibiting oxidative stress, neuronal apoptosis, and inflammation via activating the nuclear factor erythroid 2 related factor 2/heme oxygenase-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Luo,Zhangli Bao,Mingjian Zhou,Yuxin Chen,Zhaoxi Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Immunoprecipitation - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Western blot - Anti-Caveolin-3 antibody [EPR18975] - BSA and Azide free (AB250637)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise