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AB289555

Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free

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Rabbit Recombinant Multiclonal Caveolin-3 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

View Alternative Names

Caveolin-3, M-caveolin, CAV3

17 Images
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic staining in RD cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized RD (Human muscle spindle cells and large multinucleated cells) cells labelling Caveolin-3 with ab289544 at 1/50 dilution (1 μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Negative control : no staining on human kidney.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IP

Supplier Data

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Caveolin-3 was immunoprecipitated from 0.35 mg human heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.

Lane 1 : Human heart lysate 10 μg

Lane 2 : ab289544 IP in human heart lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289544 in human heart lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-rm1042-caveolae-marker-ab289544'>ab289544</a>)

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Negative control : no staining on mouse kidney.

Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) differentiated to muscle for 7 days (Right) / Undifferentiated C2C12 cells (Left). Cells labelling Caveolin-3 with ab289544 at 1/500 dilution (0.1 μg)/ Left and Right. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 μg/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Negative control : no staining on rat kidney.

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IP

Supplier Data

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Caveolin-3 was immunoprecipitated from 0.35 mg mouse heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse heart lysate 10 μg

Lane 2 : ab289544 IP in mouse heart lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289544 in mouse heart lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

Dimerized Caveolin-3 was observed at around 34kDa. (PMID : 21362625)

All lanes:

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-rm1042-caveolae-marker-ab289544'>ab289544</a>)

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • IP

Supplier Data

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Caveolin-3 was immunoprecipitated from 0.35 mg rat heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.

Lane 1 : Rat heart lysate 10 μg

Lane 2 : ab289544 IP in rat heart lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289544 in rat heart lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

Dimerized Caveolin-3 was observed at around 34kDa. (PMID : 21362625)

All lanes:

Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-rm1042-caveolae-marker-ab289544'>ab289544</a>)

Predicted band size: 17 kDa

false

Western blot - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • WB

Lab

Western blot - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Dimerized Caveolin-3 was observed at around 34kDa. (PMID : 21362625)

Exposure time : 100 seconds

All lanes:

Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-rm1042-caveolae-marker-ab289544'>ab289544</a>) at 1/1000 dilution

Lane 1:

RD (Human muscle spindle cells and large multinucleated cells) whole cell lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Western blot - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)
  • WB

Lab

Western blot - Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free (AB289555)

This data was developed using ab289544, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST **Negative control** : Kidney (PMID : 8567687). Higher molecular bands could be sumoylated Caveolin-3. (PMID : 21362625) Exposure time : 15 seconds

All lanes:

Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (<a href='/en-us/products/primary-antibodies/caveolin-3-antibody-rm1042-caveolae-marker-ab289544'>ab289544</a>) at 1/1000 dilution

Lane 1:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 2:

C2C12 differentiated to muscle for 7 days whole cell lysate at 20 µg

Lane 3:

Human heart lysate at 20 µg

Lane 4:

Human kidney lysate at 20 µg

Lane 5:

Mouse heart lysate at 20 µg

Lane 6:

Mouse kidney lysate at 20 µg

Lane 7:

Rat heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

  • Unconjugated

    Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1042

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

IP, Flow Cyt (Intra), IHC-Fr, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

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Primary Antibodies

AB8227

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab289555 is the carrier free version of ab289544.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-3 also referred to as 'caveolin' 'caveolae' and 'CAV3' is a protein with an approximate mass of 17 kDa. It acts as a structural component of caveolae small invaginations in the plasma membrane. Caveolin-3 is expressed majorly in muscle cells including cardiac skeletal and smooth muscle tissues. As a distinguishing marker for caveolae it plays an essential role in the formation and stabilization of these structures.
Biological function summary

Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.

Pathways

Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.

Caveolin-3 mutations have been associated with a range of muscle-related disorders including limb-girdle muscular dystrophy and hypertrophic cardiomyopathy. These conditions often arise due to alterations in muscle cell signaling and integrity. Caveolin-3 also shares connections with proteins like dystrophin an important player in maintaining muscle cell structure and its dysfunction can exacerbate these muscular disorders. Understanding these interactions offers insights into potential therapeutic targets for managing related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed : 19262564).
See full target information CAV3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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