Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
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(1 Publication)
Rabbit Recombinant Multiclonal Caveolin-3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
View Alternative Names
Caveolin-3, M-caveolin, CAV3
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic staining in RD cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized RD (Human muscle spindle cells and large multinucleated cells) cells labelling Caveolin-3 with ab289544 at 1/50 dilution (1 μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control : no staining on human kidney.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Caveolin-3 was immunoprecipitated from 0.35 mg human heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : Human heart lysate 10 μg
Lane 2 : ab289544 IP in human heart lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289544 in human heart lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds
All lanes:
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Predicted band size: 17 kDa
Observed band size: 17 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control : no staining on mouse kidney.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) differentiated to muscle for 7 days (Right) / Undifferentiated C2C12 cells (Left). Cells labelling Caveolin-3 with ab289544 at 1/500 dilution (0.1 μg)/ Left and Right. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 μg/mL) dilution.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control : no staining on rat kidney.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Caveolin-3 was immunoprecipitated from 0.35 mg mouse heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.
Lane 1 : Mouse heart lysate 10 μg
Lane 2 : ab289544 IP in mouse heart lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289544 in mouse heart lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 8 seconds
Dimerized Caveolin-3 was observed at around 34kDa. (PMID : 21362625)
All lanes:
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Predicted band size: 17 kDa
Observed band size: 17 kDa
false
- IP
Supplier Data
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Caveolin-3 was immunoprecipitated from 0.35 mg rat heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.
Lane 1 : Rat heart lysate 10 μg
Lane 2 : ab289544 IP in rat heart lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289544 in rat heart lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 8 seconds
Dimerized Caveolin-3 was observed at around 34kDa. (PMID : 21362625)
All lanes:
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Predicted band size: 17 kDa
false
- WB
Lab
Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Dimerized Caveolin-3 was observed at around 34kDa. (PMID : 21362625)
Exposure time : 100 seconds
All lanes:
Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544) at 1/1000 dilution
Lane 1:
RD (Human muscle spindle cells and large multinucleated cells) whole cell lysate at 20 µg
Lane 2:
Human skeletal muscle lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
false
- WB
Lab
Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Negative control : Kidney (PMID : 8567687).
Higher molecular bands could be sumoylated Caveolin-3. (PMID : 21362625)
Exposure time : 15 seconds.
All lanes:
Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544) at 1/1000 dilution
Lane 1:
C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 2:
C2C12 differentiated to muscle for 7 days whole cell lysate at 20 µg
Lane 3:
Human heart lysate at 20 µg
Lane 4:
Human kidney lysate at 20 µg
Lane 5:
Mouse heart lysate at 20 µg
Lane 6:
Mouse kidney lysate at 20 µg
Lane 7:
Rat heart lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
false
Related conjugates and formulations (1)
-
Anti-Caveolin-3 antibody [RM1042] - BSA and Azide free
Reactivity data
Product details
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties and storage information
Form
Purification technique
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Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.
Pathways
Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.
Product protocols
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Target data
Publications (1)
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Experimental and therapeutic medicine 27:97 PubMed38356676
2024
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