Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)

Rabbit Recombinant Multiclonal Caveolin-3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (AB289544), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Tested	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info 1/30	Notes -
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info 1/5000	Notes -
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information CAV3

Function

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed:19262564).

Alternative names

Caveolin-3, M-caveolin, CAV3

Recommended products

Rabbit Recombinant Multiclonal Caveolin-3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1042
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Caveolin-3 also referred to as 'caveolin' 'caveolae' and 'CAV3' is a protein with an approximate mass of 17 kDa. It acts as a structural component of caveolae small invaginations in the plasma membrane. Caveolin-3 is expressed majorly in muscle cells including cardiac skeletal and smooth muscle tissues. As a distinguishing marker for caveolae it plays an essential role in the formation and stabilization of these structures.

Biological function summary

Caveolae perform vital functions in cellular signaling and endocytosis. Caveolin-3 assists in various signaling pathways within muscle cells and interacts with other caveolae markers to facilitate signal transduction. It often forms a complex with other caveolin proteins such as caveolin-1 creating an environment conducive for the embedding of signaling molecules. This protein also modulates the trafficking and function of certain ion channels and receptors impacting muscle physiology.

Pathways

Caveolin-3 significantly influences pathways concerned with muscle function and signal transduction. In particular it involves itself with the nitric oxide (NO) signaling pathway and beta-adrenergic signaling both critical to the contractile functions of muscle tissues. It closely interacts with proteins such as endothelial nitric oxide synthase (eNOS) and components of the G-protein coupled receptor family indicating its pivotal role in mediating physiological responses to stimuli.

Associated diseases and disorders

Caveolin-3 mutations have been associated with a range of muscle-related disorders including limb-girdle muscular dystrophy and hypertrophic cardiomyopathy. These conditions often arise due to alterations in muscle cell signaling and integrity. Caveolin-3 also shares connections with proteins like dystrophin an important player in maintaining muscle cell structure and its dysfunction can exacerbate these muscular disorders. Understanding these interactions offers insights into potential therapeutic targets for managing related pathologies.

Product promise

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17 product images

Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Caveolin-3 Western blot staining using rabbit Anti-Caveolin-3 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Kidney (PMID: 8567687).
Higher molecular bands could be sumoylated Caveolin-3. (PMID: 21362625)
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544) at 1/1000 dilution
Lane 1: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 2: C2C12 differentiated to muscle for 7 days whole cell lysate at 20 µg
Lane 3: Human heart lysate at 20 µg
Lane 4: Human kidney lysate at 20 µg
Lane 5: Mouse heart lysate at 20 µg
Lane 6: Mouse kidney lysate at 20 µg
Lane 7: Rat heart lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/5000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Caveolin-3 was immunoprecipitated from 0.35 mg mouse heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/10000 dilution.
Lane 1: Mouse heart lysate 10 μg
Lane 2: ab289544 IP in mouse heart lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab289544 in mouse heart lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
All lanes: Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic staining in RD cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 μg/mL) dilution.
Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized RD (Human muscle spindle cells and large multinucleated cells) cells labelling Caveolin-3 with ab289544 at 1/50 dilution (1 μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Caveolin-3 Western blot staining using rabbit Anti-Caveolin-3 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
Exposure time: 100 seconds
All lanes: Western blot - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544) at 1/1000 dilution
Lane 1: RD (Human muscle spindle cells and large multinucleated cells) whole cell lysate at 20 µg
Lane 2: Human skeletal muscle lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control: no staining on human kidney.
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Caveolin-3 was immunoprecipitated from 0.35 mg human heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human heart lysate 10 μg
Lane 2: ab289544 IP in human heart lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab289544 in human heart lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
All lanes: Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control: no staining on mouse kidney.
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Caveolin-3 was immunoprecipitated from 0.35 mg rat heart lysate 10 μg with ab289544 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/10000 dilution.
Lane 1: Rat heart lysate 10 μg
Lane 2: ab289544 IP in rat heart lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab289544 in rat heart lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
All lanes: Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] - Caveolae Marker (ab289544)
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control: no staining on rat kidney.
Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) differentiated to muscle for 7 days (Right) / Undifferentiated C2C12 cells (Left). Cells labelling Caveolin-3 with ab289544 at 1/500 dilution (0.1 μg)/ Left and Right. Goat Anti-Rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.