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Rabbit Polyclonal CBF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus.

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Images

Western blot - Anti-CBF antibody (AB176579), expandable thumbnail
  • Immunoprecipitation - Anti-CBF antibody (AB176579), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q03701

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Chimpanzee
Predicted
Predicted
Gorilla
Predicted
Predicted
Orangutan
Predicted
Predicted
Rhesus monkey
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
2-10 µg
Notes

-

Predicted
Predicted

Species
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000 - 1/10000
Notes

-

Predicted
Predicted

Species
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Stimulates transcription from the HSP70 promoter.

Alternative names

Recommended products

Rabbit Polyclonal CBF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q03701
Purification technique
Affinity purification Immunogen
Concentration
Loading...
Purification notes

ab176579 was affinity purified using an epitope specific to CBF immobilized on solid support.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Core Binding Factor (CBF) also known sometimes as CBF protein is a transcription factor that plays an important role in the regulation of gene expression. CBF is composed of two subunits: the alpha (CBFα) and beta (CBFβ) subunits. CBFβ does not bind DNA on its own but enhances the DNA binding ability of CBFα. The mass of the CBF complex is not fixed and varies depending on the isoform expressions and modifications. It is commonly expressed in hematopoietic cells and bone tissue where it influences the transcriptional control necessary for cell differentiation and development.

Biological function summary

CBF participates in the regulation of critical transcriptional pathways. It's known primarily for its involvement in the binding of DNA motifs working closely with RUNX family proteins to modulate the transcription of genes pivotal for cell cycle and differentiation. CBF functions within a complex most notably with the RUNX family contributing significantly to hematopoietic development and bone formation. Its regulatory mechanisms have broad implications given its influence on essential cellular functions through transcription regulation.

Pathways

CBF integrates into significant biological pathways. Notably it is involved in the RUNX-mediated pathway which is important for hematopoiesis as well as osteogenesis. Within these pathways CBF interacts closely with RUNX1 and RUNX2 to regulate gene transcription required for normal functioning and developmental processes. The interplay and precise regulation of these proteins within these pathways highlight their importance in maintaining cellular homeostasis and proper lineage specification.

Associated diseases and disorders

CBF's misregulation is linked with leukemia and bone-related disorders. In leukemia especially acute myeloid leukemia chromosomal translocations affecting RUNX1 or CBFβ subunits result in fusion proteins that can disrupt normal gene regulation leading to unregulated cell proliferation. In bone disorders abnormalities in CBF expression or function can affect RUNX2-related pathways impacting bone density and formation evident in conditions like cleidocranial dysplasia. The association of CBF with these disorders highlights its significance in maintaining not only cellular differentiation but also organismal health.

Product promise

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2 product images

  • Western blot - Anti-CBF antibody (ab176579), expandable thumbnail

    Western blot - Anti-CBF antibody (ab176579)

    All lanes: Western blot - Anti-CBF antibody (ab176579) at 0.04 µg/mL

    Lane 1: HeLa whole cell lysates at 50 µg

    Lane 2: HeLa whole cell lysates at 15 µg

    Lane 3: HeLa whole cell lysates at 5 µg

    Lane 4: 293T whole cell lysates at 50 µg

    Developed using the ECL technique.

    Predicted band size: 121 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-CBF antibody (ab176579), expandable thumbnail

    Immunoprecipitation - Anti-CBF antibody (ab176579)

    Detection of CBF in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176579 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab176579 was used at 1 μg/ml. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

    All lanes: Immunoprecipitation - Anti-CBF antibody (ab176579)

    Predicted band size: 121 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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