Rabbit Polyclonal CBF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CEBPZ aa 1000 to C-terminus.
View Alternative Names
CBF2, CEBPZ, CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor
- IP
Supplier Data
Immunoprecipitation - Anti-CBF antibody (AB176579)
Detection of CBF in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176579 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab176579 was used at 1 μg/ml. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-CBF antibody (ab176579)
Predicted band size: 121 kDa
false
- WB
Supplier Data
Western blot - Anti-CBF antibody (AB176579)
All lanes:
Western blot - Anti-CBF antibody (ab176579) at 0.04 µg/mL
Lane 1:
HeLa whole cell lysates at 50 µg
Lane 2:
HeLa whole cell lysates at 15 µg
Lane 3:
HeLa whole cell lysates at 5 µg
Lane 4:
293T whole cell lysates at 50 µg
Predicted band size: 121 kDa
true
Exposure time: 30s
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- 0
- 0
Reactivity data
Properties and storage information
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Supplementary information
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Biological function summary
CBF participates in the regulation of critical transcriptional pathways. It's known primarily for its involvement in the binding of DNA motifs working closely with RUNX family proteins to modulate the transcription of genes pivotal for cell cycle and differentiation. CBF functions within a complex most notably with the RUNX family contributing significantly to hematopoietic development and bone formation. Its regulatory mechanisms have broad implications given its influence on essential cellular functions through transcription regulation.
Pathways
CBF integrates into significant biological pathways. Notably it is involved in the RUNX-mediated pathway which is important for hematopoiesis as well as osteogenesis. Within these pathways CBF interacts closely with RUNX1 and RUNX2 to regulate gene transcription required for normal functioning and developmental processes. The interplay and precise regulation of these proteins within these pathways highlight their importance in maintaining cellular homeostasis and proper lineage specification.
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Publications (2)
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Plant biotechnology journal 22:1402-1416 PubMed38163285
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature 552:126-131 PubMed29186125
2017
Applications
Unspecified application
Species
Unspecified reactive species
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