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AB89034

Anti-CBF antibody

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(1 Publication)

Mouse Polyclonal CBF antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CEBPZ.

View Alternative Names

CBF2, CEBPZ, CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CBF antibody (AB89034)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CBF antibody (AB89034)

ab89034, at 10 μg/ml, staining CBF in Hela cell by Immunofluorescence.

Western blot - Anti-CBF antibody (AB89034)
  • WB

Unknown

Western blot - Anti-CBF antibody (AB89034)

All lanes:

Western blot - Anti-CBF antibody (ab89034) at 1 µg/mL

All lanes:

K-562 cell lysate at 50 µg

Predicted band size: 121 kDa

Observed band size: 131 kDa

false

Western blot - Anti-CBF antibody (AB89034)
  • WB

Unknown

Western blot - Anti-CBF antibody (AB89034)

All lanes:

Western blot - Anti-CBF antibody (ab89034) at 1 µg/mL

Lane 1:

CEBPZ transfected 293T cell lysate at 25 µg

Lane 2:

non transfected 293T cell lysate at 25 µg

Predicted band size: 121 kDa

Observed band size: 250 kDa

false

Western blot - Anti-CBF antibody (AB89034)
  • WB

Unknown

Western blot - Anti-CBF antibody (AB89034)

All lanes:

Western blot - Anti-CBF antibody (ab89034) at 1 µg/mL

All lanes:

NIH/3T3 cell lysate at 50 µg

Predicted band size: 121 kDa

Observed band size: 150 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CEBPZ.

Q03701

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Core Binding Factor (CBF) also known sometimes as CBF protein is a transcription factor that plays an important role in the regulation of gene expression. CBF is composed of two subunits: the alpha (CBFα) and beta (CBFβ) subunits. CBFβ does not bind DNA on its own but enhances the DNA binding ability of CBFα. The mass of the CBF complex is not fixed and varies depending on the isoform expressions and modifications. It is commonly expressed in hematopoietic cells and bone tissue where it influences the transcriptional control necessary for cell differentiation and development.
Biological function summary

CBF participates in the regulation of critical transcriptional pathways. It's known primarily for its involvement in the binding of DNA motifs working closely with RUNX family proteins to modulate the transcription of genes pivotal for cell cycle and differentiation. CBF functions within a complex most notably with the RUNX family contributing significantly to hematopoietic development and bone formation. Its regulatory mechanisms have broad implications given its influence on essential cellular functions through transcription regulation.

Pathways

CBF integrates into significant biological pathways. Notably it is involved in the RUNX-mediated pathway which is important for hematopoiesis as well as osteogenesis. Within these pathways CBF interacts closely with RUNX1 and RUNX2 to regulate gene transcription required for normal functioning and developmental processes. The interplay and precise regulation of these proteins within these pathways highlight their importance in maintaining cellular homeostasis and proper lineage specification.

CBF's misregulation is linked with leukemia and bone-related disorders. In leukemia especially acute myeloid leukemia chromosomal translocations affecting RUNX1 or CBFβ subunits result in fusion proteins that can disrupt normal gene regulation leading to unregulated cell proliferation. In bone disorders abnormalities in CBF expression or function can affect RUNX2-related pathways impacting bone density and formation evident in conditions like cleidocranial dysplasia. The association of CBF with these disorders highlights its significance in maintaining not only cellular differentiation but also organismal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates transcription from the HSP70 promoter.
See full target information CEBPZ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:59 PubMed38229174

2024

Intracellular delivery of Parkin-RING0-based fragments corrects Parkin-induced mitochondrial dysfunction through interaction with SLP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Zanon,Marianna Guida,Alexandros A Lavdas,Corrado Corti,Maria Paulina Castelo Rueda,Alessandro Negro,Peter P Pramstaller,Francisco S Domingues,Andrew A Hicks,Irene Pichler
View all publications
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