Rabbit Recombinant Monoclonal CBF antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-CBF antibody [EPR27441-85] (AB314319), expandable thumbnail](https://content.abcam.com/products/images/cbf-antibody-epr27441-85-ab314319--western-blot-img421001.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CBF antibody [EPR27441-85] (AB314319)")
![Western blot - Anti-CBF antibody [EPR27441-85] (AB314319), expandable thumbnail](https://content.abcam.com/products/images/cbf-antibody-epr27441-85-ab314319--western-blot-img421000.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CBF antibody [EPR27441-85] (AB314319)")
![Western blot - Anti-CBF antibody [EPR27441-85] (AB314319), expandable thumbnail](https://content.abcam.com/products/images/cbf-antibody-epr27441-85-ab314319--western-blot-img420999.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CBF antibody [EPR27441-85] (AB314319)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Alternative names
CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor, Cebpa-rs1, Cebpz, Cbf2
Recommended products
Rabbit Recombinant Monoclonal CBF antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Alternative names
CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor, Cebpa-rs1, Cebpz, Cbf2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR27441-85
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CBF antibody [EPR27441-85] (ab314319), expandable thumbnail](https://content.abcam.com/products/images/cbf-antibody-epr27441-85-ab314319--western-blot-img421001.jpg "Western blot - Anti-CBF antibody [EPR27441-85] (ab314319)")
Western blot - Anti-CBF antibody [EPR27441-85] (ab314319)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-CBF antibody [EPR27441-85] (ab314319) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 121 kDa
Exposure time: 180s
![Western blot - Anti-CBF antibody [EPR27441-85] (ab314319), expandable thumbnail](https://content.abcam.com/products/images/cbf-antibody-epr27441-85-ab314319--western-blot-img421000.jpg "Western blot - Anti-CBF antibody [EPR27441-85] (ab314319)")
Western blot - Anti-CBF antibody [EPR27441-85] (ab314319)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-CBF antibody [EPR27441-85] (ab314319) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Lane 5: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 6: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 121 kDa
Exposure time: 37s
![Western blot - Anti-CBF antibody [EPR27441-85] (ab314319), expandable thumbnail](https://content.abcam.com/products/images/cbf-antibody-epr27441-85-ab314319--western-blot-img420999.jpg "Western blot - Anti-CBF antibody [EPR27441-85] (ab314319)")
Western blot - Anti-CBF antibody [EPR27441-85] (ab314319)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-CBF antibody [EPR27441-85] (ab314319) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 10 µg
Lane 2: NIH/3T3 transfected with siRNA specifically targeting CBF whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 121 kDa
Exposure time: 37s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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