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AB314320

Anti-CBF antibody [EPR27441-85] - BSA and Azide free

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Rabbit Recombinant Monoclonal CBF antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Cbf2, Cebpa-rs1, Cebpz, CCAAT/enhancer-binding protein zeta, CCAAT-box-binding transcription factor, CBF, CCAAT-binding factor

3 Images
Western blot - Anti-CBF antibody [EPR27441-85] - BSA and Azide free (AB314320)
  • WB

Supplier Data

Western blot - Anti-CBF antibody [EPR27441-85] - BSA and Azide free (AB314320)

This data was developed using ab314319, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CBF antibody [EPR27441-85] (<a href='/en-us/products/primary-antibodies/cbf-antibody-epr27441-85-ab314319'>ab314319</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 5:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 6:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 121 kDa

false

Exposure time: 37s

Western blot - Anti-CBF antibody [EPR27441-85] - BSA and Azide free (AB314320)
  • WB

Supplier Data

Western blot - Anti-CBF antibody [EPR27441-85] - BSA and Azide free (AB314320)

This data was developed using ab314319, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CBF antibody [EPR27441-85] (<a href='/en-us/products/primary-antibodies/cbf-antibody-epr27441-85-ab314319'>ab314319</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 121 kDa

false

Exposure time: 180s

Western blot - Anti-CBF antibody [EPR27441-85] - BSA and Azide free (AB314320)
  • WB

Supplier Data

Western blot - Anti-CBF antibody [EPR27441-85] - BSA and Azide free (AB314320)

This data was developed using ab314319, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CBF antibody [EPR27441-85] (<a href='/en-us/products/primary-antibodies/cbf-antibody-epr27441-85-ab314319'>ab314319</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 10 µg

Lane 2:

NIH/3T3 transfected with siRNA specifically targeting CBF whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 121 kDa

false

Exposure time: 37s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27441-85

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314320 is the carrier-free version of ab314319.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Core Binding Factor (CBF) also known sometimes as CBF protein is a transcription factor that plays an important role in the regulation of gene expression. CBF is composed of two subunits: the alpha (CBFα) and beta (CBFβ) subunits. CBFβ does not bind DNA on its own but enhances the DNA binding ability of CBFα. The mass of the CBF complex is not fixed and varies depending on the isoform expressions and modifications. It is commonly expressed in hematopoietic cells and bone tissue where it influences the transcriptional control necessary for cell differentiation and development.
Biological function summary

CBF participates in the regulation of critical transcriptional pathways. It's known primarily for its involvement in the binding of DNA motifs working closely with RUNX family proteins to modulate the transcription of genes pivotal for cell cycle and differentiation. CBF functions within a complex most notably with the RUNX family contributing significantly to hematopoietic development and bone formation. Its regulatory mechanisms have broad implications given its influence on essential cellular functions through transcription regulation.

Pathways

CBF integrates into significant biological pathways. Notably it is involved in the RUNX-mediated pathway which is important for hematopoiesis as well as osteogenesis. Within these pathways CBF interacts closely with RUNX1 and RUNX2 to regulate gene transcription required for normal functioning and developmental processes. The interplay and precise regulation of these proteins within these pathways highlight their importance in maintaining cellular homeostasis and proper lineage specification.

CBF's misregulation is linked with leukemia and bone-related disorders. In leukemia especially acute myeloid leukemia chromosomal translocations affecting RUNX1 or CBFβ subunits result in fusion proteins that can disrupt normal gene regulation leading to unregulated cell proliferation. In bone disorders abnormalities in CBF expression or function can affect RUNX2-related pathways impacting bone density and formation evident in conditions like cleidocranial dysplasia. The association of CBF with these disorders highlights its significance in maintaining not only cellular differentiation but also organismal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates transcription from the HSP70 promoter.
See full target information Cebpz
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com