AB113203

Anti-CBF1 interacting corepressor/CIR1 antibody

Name: Anti-CBF1 interacting corepressor/CIR1 antibody (ab113203) | Abcam
Brand: Abcam Limited
SKU: AB113203
Price: 520 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal CBF1 interacting corepressor/CIR1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CIR1 aa 400 to C-terminus.

View Alternative Names

CIR, CIR1, Corepressor interacting with RBPJ 1, CBF1-interacting corepressor, Recepin

2 Images

Unknown

Immunoprecipitation - Anti-CBF1 interacting corepressor/CIR1 antibody (AB113203)

Detection of CBF1 interacting corepressor/CIR1 by Western Blot of Immunprecipitate.
ab113203 at 1μg/ml staining CBF1 interacting corepressor/CIR1 in HeLa whole cell lysate immunoprecipitated using ab113203 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-CBF1 interacting corepressor/CIR1 antibody (ab113203)

Predicted band size: 52 kDa

false

Unknown

Western blot - Anti-CBF1 interacting corepressor/CIR1 antibody (AB113203)

All lanes:

Western blot - Anti-CBF1 interacting corepressor/CIR1 antibody (ab113203) at 0.1 µg/mL

Lane 1:

HeLa cell Lysate at 50 µg

Lane 2:

HeLa cell Lysate at 15 µg

Lane 3:

Jurkat cell Lysate at 50 µg

Lane 4:

293T cell Lysate at 50 µg

Predicted band size: 52 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CIR1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86X95

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99.91% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CBF1 interacting corepressor also known as CIR1 is a coregulator that interacts with CBF1 an important transcriptional repressor. CIR1 forms a complex with CBF1 and other components to modulate transcriptional repression processes. The molecular mass of CIR1 is approximately 47 kDa. It expresses in various tissues throughout the body including the brain and lymphoid tissues. This broad expression pattern suggests a role in multiple cellular processes. CIR1 plays a central part in controlling gene expression by interacting with CBF1 and other transcriptional modulators.

Biological function summary

CIR1 serves as a mediator in transcriptional repression and is a part of the transducin-like enhancer of split (TLE) corepressor complex. This complex influences the activity of CBF1 by promoting chromatin modifications that lead to transcriptional repression. CIR1 contributes to finely regulating gene expression essential for maintaining cellular homeostasis. The TLE complex generally recruits CIR1 to target genes modulating the expression of genes involved in cell growth and differentiation.

Pathways

CIR1 modulates Notch signaling and Hedgehog pathways two critical signal transduction systems for cell fate determination and development. In the Notch signaling pathway CIR1 collaborates with CBF1 (also known as RBPJ or CSL) to repress target gene transcription until the pathway is activated. Interaction with Notch proteins results in pathway activation which is important for cell communication and differentiation. CIR1 indirectly affects the Hedgehog signaling pathway by influencing transcription factors that respond to this pathway highlighting its role in developmental processes and tumorigenesis.

CIR1 is associated with oncogenesis and neurological disorders. Dysregulation of Notch signaling involving CIR1 can contribute to various cancers by altering cell proliferation and apoptosis. In neurological conditions such as Alzheimer's disease CIR1's involvement in transcriptional repression may affect neuronal function by disrupting gene regulation. CIR1 also interacts with Presenilin proteins which are linked to Alzheimer's pathology indicating potential roles in disease progression. Understanding CIR1's function in these pathways can provide insights into therapeutic targets for related disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May modulate splice site selection during alternative splicing of pre-mRNAs (By similarity). Regulates transcription and acts as corepressor for RBPJ. Recruits RBPJ to the Sin3-histone deacetylase complex (HDAC). Required for RBPJ-mediated repression of transcription.

See full target information CIR1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115237 PubMed39883578

2025

G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Liana C Boraas,Mengwei Hu,Pieter Martino,Lauren Thornton,Charles E Vejnar,Gang Zhen,Longhui Zeng,Dylan M Parker,Andy L Cox,Antonio J Giraldez,Xiaolei Su,Christine Mayr,Siyuan Wang,Stefania Nicoli

Disease markers 2022:5013622 PubMed35510038

2022

Liraglutide Alleviates Diabetic Atherosclerosis through Regulating Calcification of Vascular Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Li Shi,Ming Hao,Zhou-Yun Jin,Gui-Fang Peng,Ying-Ying Tang,Hong-Yu Kuang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CBF1 interacting corepressor/CIR1 antibody

Immunoprecipitation - Anti-CBF1 interacting corepressor/CIR1 antibody (AB113203)

Western blot - Anti-CBF1 interacting corepressor/CIR1 antibody (AB113203)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise