Rabbit Polyclonal CBFb antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CBFB aa 150 to C-terminus.
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- Cell reports 36:1097252021Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabrizio Simeoni,Isabel Romero-Camarero,Francesco Camera,Fabio M R Amaral,Oliver J Sinclair,Evangelia K Papachristou,Gary J Spencer,Michael Lie-A-Ling,Georges Lacaud,Daniel H Wiseman,Jason S Carroll,Tim C P SomervaillePubMed 34551306
- Nature communications 10:20712019The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNavdeep Malik et. al.PubMed 31061501
- PLoS genetics 12:e10058842016A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Hwa Shin et. al.PubMed 26925584
- Journal of cellular physiology 229:1466-742014Core binding factor β (CBFβ) is retained in the midbody during cytokinesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCesar Lopez-Camacho,Andre J van Wijnen,Jane B Lian,Janet L Stein,Gary S SteinPubMed 24648201
- Cell stem cell 14:370-842014Direct reprogramming of human fibroblasts to functional and expandable hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengyu Huang et. al.PubMed 24582927
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CBFB aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13951
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. (Microbial infection) Following infection, hijacked by the HIV-1 Vif protein, leading to the formation a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed:22190037, PubMed:31792451, PubMed:36598981, PubMed:36754086, PubMed:37419875). The complex can also ubiquitinate APOBEC3H to some extent (PubMed:37640699). Association with HIV-1 Vif protein also inhibits the transcription coactivator activity of CBFB/CBF-beta (PubMed:22190037).
Alternative names
Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, PEA2-beta, PEBP2-beta, CBFB
Recommended products
Rabbit Polyclonal CBFb antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CBFB aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CBFB aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13951
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CBFb also known as Core-Binding Factor Subunit Beta weighs approximately 22 kDa. It plays an important role in gene regulation by stabilizing the DNA binding of core-binding factor alpha (CBFA) subunits such as RUNX proteins. CBFb does not bind DNA directly. Instead it enhances the affinity of the RUNX family for their DNA targets. CBFb is broadly expressed in various tissues with high levels observed in hematopoietic cells.
- Biological function summary
CBFb forms a complex with RUNX transcription factors facilitating their function. The complex is essential for hematopoiesis as it regulates the expression of genes critical for blood cell differentiation and proliferation. CBFb is involved in bone development influencing the formation of bone-related genes. This CBFb/RUNX complex plays a role in regulating the cell cycle and apoptosis fundamental processes for maintaining cellular integrity.
- Pathways
More than one signaling pathway includes CBFb's involvement. Particularly it participates in the Wnt signaling pathway an important player in cell differentiation. Additionally CBFb/RUNX complex regulates genes downstream in the TGF-beta signaling pathway impacting cellular proliferation and differentiation. Through these pathways CBFb is linked to proteins like SMAD3 further emphasizing its role in mediating various biological processes.
- Associated diseases and disorders
Abnormalities in CBFb function connect to acute myeloid leukemia (AML). CBFb's interaction with RUNX1 can result in a chromosomal translocation that generates the CBFb-MYH11 fusion protein which disrupts normal hematopoiesis and contributes to leukemogenesis. Moreover CBFb variations associate with cleidocranial dysplasia a skeletal disorder through its interaction with the RUNX2 protein highlighting its significance in bone development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-CBFb antibody (ab125191)
Detection of human CBFb by western blot of immunoprecipitates.
Lane 1: Sample.
Lane 2: Control IgG.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat (Human T cell leukemia cell line from peripheral blood) cells prepared using NETN lysis buffer.
Antibodies: ab125191 used for IP at 3 μg per reaction.For blotting immunoprecipitated CBFb ab125191 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-CBFb antibody (ab125191)
Predicted band size: 22 kDa
Western blot - Anti-CBFb antibody (ab125191)
Cells prepared using NETN lysis buffer.
4-12% SDS-PAGE.
All lanes: Western blot - Anti-CBFb antibody (ab125191) at 0.1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 4: TCMK (Mouse kidney epithelial cell line) whole cell lysate at 15 µg
Lane 5: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 15 µg
Predicted band size: 22 kDa
Exposure time: 30s
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