JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231345

Anti-CBFb antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal CBFb antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Cbfb.

View Alternative Names

Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, PEA2-beta, PEBP2-beta, CBFB

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBFb antibody (AB231345)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBFb antibody (AB231345)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for CBFb using ab231345 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBFb antibody (AB231345)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBFb antibody (AB231345)

Formalin-fixed, paraffin-embedded mouse stomach tissue stained for CBFb using ab231345 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-CBFb antibody (AB231345)
  • WB

Supplier Data

Western blot - Anti-CBFb antibody (AB231345)

All lanes:

Western blot - Anti-CBFb antibody (ab231345) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow whole cell lysate.

Predicted band size: 11 kDa,14 kDa,19 kDa,21 kDa,22 kDa,29 kDa,31 kDa,33 kDa,36 kDa,38 kDa

true

Western blot - Anti-CBFb antibody (AB231345)
  • WB

Supplier Data

Western blot - Anti-CBFb antibody (AB231345)

All lanes:

Western blot - Anti-CBFb antibody (ab231345) at 2 µg/mL

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 22 kDa

true

Western blot - Anti-CBFb antibody (AB231345)
  • WB

Supplier Data

Western blot - Anti-CBFb antibody (AB231345)

All lanes:

Western blot - Anti-CBFb antibody (ab231345) at 2 µg/mL

All lanes:

Recombinant mouse CBFb protein.

Predicted band size: 22 kDa

true

Western blot - Anti-CBFb antibody (AB231345)
  • WB

Supplier Data

Western blot - Anti-CBFb antibody (AB231345)

All lanes:

Western blot - Anti-CBFb antibody (ab231345) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 22 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Cbfb.

Q08024

style
left-column

Alternative Products

Primary Antibodies

AB195411

Anti-CBFb antibody - ChIP Grade

ChIP - Anti-CBFb antibody - ChIP Grade (AB195411)

  • 0
  • 0
Primary Antibodies

AB208764

Alexa Fluor® 647 Anti-CBFb antibody [EPR6322]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CBFb antibody [EPR6322] (AB208764)

  • 0
  • 0
Primary Antibodies

AB124693

Anti-CBFb antibody [EPR6321]

RabMAb|Recombinant|KO Validated

Western blot - Anti-CBFb antibody [EPR6321] (AB124693)

  • 0
  • 0
Primary Antibodies

AB133600

Anti-CBFb antibody [EPR6322]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CBFb antibody [EPR6322] (AB133600)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB23980

Anti-RUNX1 / AML1 antibody

Western blot - Anti-RUNX1 / AML1 antibody (AB23980)

  • 4
  • 14
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CBFb also known as Core-Binding Factor Subunit Beta weighs approximately 22 kDa. It plays an important role in gene regulation by stabilizing the DNA binding of core-binding factor alpha (CBFA) subunits such as RUNX proteins. CBFb does not bind DNA directly. Instead it enhances the affinity of the RUNX family for their DNA targets. CBFb is broadly expressed in various tissues with high levels observed in hematopoietic cells.
Biological function summary

CBFb forms a complex with RUNX transcription factors facilitating their function. The complex is essential for hematopoiesis as it regulates the expression of genes critical for blood cell differentiation and proliferation. CBFb is involved in bone development influencing the formation of bone-related genes. This CBFb/RUNX complex plays a role in regulating the cell cycle and apoptosis fundamental processes for maintaining cellular integrity.

Pathways

More than one signaling pathway includes CBFb's involvement. Particularly it participates in the Wnt signaling pathway an important player in cell differentiation. Additionally CBFb/RUNX complex regulates genes downstream in the TGF-beta signaling pathway impacting cellular proliferation and differentiation. Through these pathways CBFb is linked to proteins like SMAD3 further emphasizing its role in mediating various biological processes.

Abnormalities in CBFb function connect to acute myeloid leukemia (AML). CBFb's interaction with RUNX1 can result in a chromosomal translocation that generates the CBFb-MYH11 fusion protein which disrupts normal hematopoiesis and contributes to leukemogenesis. Moreover CBFb variations associate with cleidocranial dysplasia a skeletal disorder through its interaction with the RUNX2 protein highlighting its significance in bone development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.. (Microbial infection) Following infection, hijacked by the HIV-1 Vif protein, leading to the formation a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed : 22190037, PubMed : 31792451, PubMed : 36598981, PubMed : 36754086, PubMed : 37419875). The complex can also ubiquitinate APOBEC3H to some extent (PubMed : 37640699). Association with HIV-1 Vif protein also inhibits the transcription coactivator activity of CBFB/CBF-beta (PubMed : 22190037).
See full target information CBFB
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:61 PubMed40708830

2025

Microfibrillar-associated protein-2 facilitates aggressive progression of oral squamous cell carcinoma cells through Kelch-like E3 ubiquitin ligase-associated protein 1/nuclear factor erythroid 2-related factor 2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wanzhen Yang,Junyi Tu,Kaixi Dai,Kemin Jia

Molecules and cells 46:231-244 PubMed36625319

2023

RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Ding,Xiaoliang Mei,Lintao Li,Peng Fang,Ting Guo,Jianning Zhao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com