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Rabbit Recombinant Monoclonal CBL antibody. C-terminal. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

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Images

Western blot - Anti-CBL antibody [YE323] - C-terminal (AB32027), expandable thumbnail
  • Western blot - Anti-CBL antibody [YE323] - C-terminal (AB32027), expandable thumbnail
  • Western blot - Anti-CBL antibody [YE323] - C-terminal (AB32027), expandable thumbnail
  • Western blot - Anti-CBL antibody [YE323] - C-terminal (AB32027), expandable thumbnail
  • Western blot - Anti-CBL antibody [YE323] - C-terminal (AB32027), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Expected
Not recommended
Tested
Expected
Rat
Expected
Not recommended
Tested
Expected
Chicken
Predicted
Not recommended
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1/100

Expected
Expected

Species
Mouse
Dilution info
1/50
Notes

For unpurified use at 1/100

Species
Rat
Dilution info
1/50
Notes

For unpurified use at 1/100

Predicted
Predicted

Species
Chicken
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Chicken
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

For unpurified use at 1/5000

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/5000

Species
Rat
Dilution info
1/1000
Notes

For unpurified use at 1/5000

Predicted
Predicted

Species
Chicken
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

For unpurified use at 1/100 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
1/30
Notes

For unpurified use at 1/100 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
1/30
Notes

For unpurified use at 1/100 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Chicken
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of many signaling pathways by mediating ubiquitination of cell surface receptors (PubMed:10514377, PubMed:11896602, PubMed:14661060, PubMed:14739300, PubMed:15190072, PubMed:17509076, PubMed:18374639, PubMed:19689429, PubMed:21596750, PubMed:28381567). Accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed:10514377, PubMed:14661060, PubMed:14739300, PubMed:17094949, PubMed:17509076, PubMed:17974561). Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and mediates their ubiquitination to terminate signaling (PubMed:15190072, PubMed:18374639, PubMed:21596750). Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation (PubMed:11896602). Ubiquitinates EGFR and SPRY2 (PubMed:17094949, PubMed:17974561). Ubiquitinates NECTIN1 following association between NECTIN1 and herpes simplex virus 1/HHV-1 envelope glycoprotein D, leading to NECTIN1 removal from cell surface (PubMed:28381567). Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis (PubMed:15190072, PubMed:18374639). Essential for osteoclastic bone resorption (PubMed:14739300). The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function (PubMed:14739300). May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).

Alternative names

CBL2, RNF55, CBL, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, Signal transduction protein CBL

Recommended products

Rabbit Recombinant Monoclonal CBL antibody. C-terminal. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
YE323
Purification technique
Affinity purification Protein A
Epitope
ab32027 reacts with an epitope located in the C terminal region of CBL.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The CBL protein also known as C-cbl or Casitas B-lineage lymphoma proto-oncogene serves as an E3 ubiquitin-protein ligase. This protein with a molecular mass of approximately 120-130 kDa plays a critical role in the regulation of receptor tyrosine kinases. It facilitates the polyubiquitination of activated receptor proteins targeting them for degradation in the proteasome. CBL is predominantly expressed in various tissues including the hematopoietic system. It is a significant component in the control of cell signaling and helps maintain cellular homeostasis.

Biological function summary

The CBL protein acts as a negative regulator for several signaling pathways. It integrates into signaling complexes and modulates the activation of pathways by interacting with other proteins. CBL does not act alone; it commonly forms complexes with proteins such as Grb2 and p85 influencing downstream signaling influence in pathways. This regulation prevents excessive activation of signals that can lead to cellular proliferation or differentiation abnormally.

Pathways

CBL integrates into the ubiquitin-proteasome system and the Ras signaling pathway. Within these pathways CBL works to modulate cell surface receptors ensuring that signaling threshold is not surpassed. The importance of CBL in the Ras signaling pathway is highlighted by its interactions with proteins like Ras-GAP and SHP2. Through these interactions CBL controls the rate of signal transduction affecting cellular responses such as growth survival and motility.

Associated diseases and disorders

CBL involvement has been linked to several forms of cancer and immune deficiencies. Mutations or dysregulation of the CBL gene can lead to myelodysplastic syndromes and acute myeloid leukemia. In these conditions the interaction between CBL and other proteins such as JAK2 becomes dysregulated driving abnormal cell growth and survival. Furthermore CBL’s failure to properly degrade tyrosine kinase receptors can contribute to enhanced signaling fostering oncogenic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027), expandable thumbnail

    Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    All lanes: Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027) at 1/1000 dilution

    Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

    Lane 2: Rat testis lysate at 20 µg

    Lane 3: Rat thymus lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Predicted band size: 100 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027), expandable thumbnail

    Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    Lanes 1-4: Merged signal (red and green). Green - ab32027 observed at 110 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    Unpurified ab32027 Anti-CBL antibody [YE323] - C-terminal was shown to specifically react with CBL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human CBL knockout HEK-293T cell line ab267245 (knockout cell lysate Human CBL knockout HEK-293T cell lysate ab257200) was used. Wild-type and CBL knockout samples were subjected to SDS-PAGE. ab32027 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: CBL knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human CBL knockout HEK-293T cell line (Human CBL knockout HEK-293T cell line ab267245)

    Lane 3: THP-1 cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 100 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027), expandable thumbnail

    Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: CBL knockout HAP1 whole cell lysate (20 μg)
    Lane 3: THP1 whole cell lysate (20 μg)
    Lane 4: Raji whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32027 observed at 100 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    Unpurified ab32027 was shown to specifically react with CBL in wild-type HAP1 cells as signal was lost in CBL knockout cells. Wild-type and CBL knockout samples were subjected to SDS-PAGE. ab32027 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    Predicted band size: 100 kDa

  • Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027), expandable thumbnail

    Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 30 seconds

    All lanes: Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027) at 1/1000 dilution

    Lane 1: WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 10 µg

    Lane 2: F9 (Mouse embryonal carcinoma epithelial cell) cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) cell lysate at 10 µg

    Lane 4: Mouse thymus lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 100 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027), expandable thumbnail

    Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    Western blot: Anti-CBL antibody [YE323] (ab32027) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab32027 was shown to bind specifically to CBL. A band was observed at 105 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in CBL knockout cell line. To generate this image, wild-type and CBL knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-CBL antibody [YE323] - C-terminal (ab32027) at 1/1000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: CBL knockout HCT 116 cell lysate at 20 µg

    Lane 2: Western blot - Human CBL knockout HCT116 cell line (Human CBL knockout HCT116 cell line ab287255)

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 105 kDa

  • Flow Cytometry (Intracellular) - Anti-CBL antibody [YE323] - C-terminal (ab32027), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CBL antibody [YE323] - C-terminal (ab32027)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CBL with purified ab32027 at 1/30 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Product protocols

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