Anti-CBLB antibody [EPR28082-32] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-CBLB antibody [EPR28082-32] - BSA and Azide free (AB315022)

This data was developed using ab315021, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 38 kDa is unknown.

The identity of the band higher than 250 kDa is unknown.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-CBLB antibody [EPR28082-32] (<a href='/en-us/products/primary-antibodies/cblb-antibody-epr28082-32-ab315021'>ab315021</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 125 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CBLB antibody [EPR28082-32] - BSA and Azide free (AB315022)

This data was developed using ab315021, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab315021 was shown to bind specifically to CBLB. A band was observed at 125 kDa in wild-type Hela cell lysates with whereas no signal observed at this size in CBLB knockout cell line ab264711 (knockout cell lysate ab258342).

The identity of the band at 38 kDa is unknown.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CBLB antibody [EPR28082-32] (<a href='/en-us/products/primary-antibodies/cblb-antibody-epr28082-32-ab315021'>ab315021</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CBLB knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-cblb-knockout-hela-cell-lysate-ab258342'>ab258342</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 125 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CBLB antibody [EPR28082-32] - BSA and Azide free (AB315022)

This data was developed using ab315021, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 38 kDa is unknown.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-CBLB antibody [EPR28082-32] (<a href='/en-us/products/primary-antibodies/cblb-antibody-epr28082-32-ab315021'>ab315021</a>) at 1/1000 dilution

Lane 1:

Rat liver lysate at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Lane 3:

Mouse testis lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 125 kDa

false

Exposure time: 180s