Rabbit Polyclonal CBP80 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NCBP1 aa 100-250.
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- BioMed research international 2022:69183962022hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangwei Jiang,Mingxuan Qu,Lili Kong,Xiaoming Song,Shanhao JiangPubMed 35909488
- Frontiers in surgery 9:8939772022N7-Methylguanosine Regulatory Genes Profoundly Affect the Prognosis, Progression, and Antitumor Immune Response of Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKexiang Zhou,Jiaqun Yang,Xiaoyan Li,Wei Xiong,Pengbin Zhang,Xuqing ZhangPubMed 35784919
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NCBP1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q09161
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).
Alternative names
Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein, CBP80, NCBP 80 kDa subunit, NCBP1, CBP80, NCBP
Recommended products
Rabbit Polyclonal CBP80 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NCBP1 aa 100-250.
Alternative names
Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein, CBP80, NCBP 80 kDa subunit, NCBP1, CBP80, NCBP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NCBP1 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q09161
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CBP80 also known as cap-binding protein 80 is an important component of the cap-binding complex (CBC) with a mass of about 80 kDa. This protein primarily binds to the 5' cap structure of newly synthesized pre-mRNA in eukaryotic cells. CBP80 often associates with CBP20 and together they form the CBC. It facilitates the processing of pre-mRNA and is mainly expressed in the nucleus of cells. CBP80 is integral in stabilizing RNA transcripts during their maturation.
- Biological function summary
CBP80 plays a role in the initiation of mRNA processing events. As a part of the CBC it influences RNA splicing export and translation initiation. It participates in early mRNA export from the nucleus to the cytoplasm and is essential in quality control mechanisms that ensure accurate mRNA translation. The CBP80-CBP20 complex also impacts nonsense-mediated mRNA decay (NMD) a process that degrades faulty mRNAs preventing the production of truncated proteins.
- Pathways
CBP80 integrates into the mRNA surveillance pathway where it works closely with other key proteins such as UPF1 UPF2 and UPF3 in the NMD pathway. These interactions ensure erroneous mRNA is efficiently targeted and degraded. Additionally CBP80 plays a minor role in the pathway of general mRNA export where it cooperates with export factors like CRM1. Its involvement in these pathways highlights its role in maintaining the fidelity of gene expression.
- Associated diseases and disorders
CBP80's malfunction has been linked to certain cancer types and neurodevelopmental disorders. Altered activity of CBP80 can lead to the accumulation of aberrant mRNA transcripts which contributes to oncogenesis. In some neurodevelopmental disorders mutations affecting CBP80 or its interacting partner CBP20 can disrupt normal neural development. This suggests that CBP80 not only maintains normal cell function but also prevents the development of disease through its regulation of gene expression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-CBP80 antibody (ab154532)
7.5% SDS PAGE
All lanes: Western blot - Anti-CBP80 antibody (ab154532) at 1/2000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBP80 antibody (ab154532)
Immunohistochemical analysis of paraffin-embedded OVCA xenograft tissue labeling CBP80 with ab154532 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CBP80 antibody (ab154532)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling CBP80 with ab154532 at 1/200 dilution. Lower panel merged with DNA probe.
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