JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250271

Anti-CBR3 antibody [EPR14129] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal CBR3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SDR21C2, CBR3, Carbonyl reductase [NADPH] 3, NADPH-dependent carbonyl reductase 3, Quinone reductase CBR3, Short chain dehydrogenase/reductase family 21C member 2

2 Images
Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] - BSA and Azide free (AB250271)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] - BSA and Azide free (AB250271)

This data was developed using ab180939, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-CBR3 antibody [EPR14129] - BSA and Azide free (AB250271)
  • WB

Supplier Data

Western blot - Anti-CBR3 antibody [EPR14129] - BSA and Azide free (AB250271)

This data was developed using ab180939, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CBR3 antibody [EPR14129] (<a href='/en-us/products/primary-antibodies/cbr3-antibody-epr14129-ab180939'>ab180939</a>) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14129

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab250271 is the carrier-free version of ab180939.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme carbonyl reductase 3 commonly known as CBR3 is a member of the short-chain dehydrogenase/reductase superfamily. With a molecular mass of approximately 31 kDa CBR3 catalyzes the reduction of carbonyl compounds into alcohols using NADPH as a cofactor. It is expressed in various tissues including liver kidney and heart where it plays a role in xenobiotic metabolism and the detoxification processes by reducing carbonyl-containing compounds.
Biological function summary

CBR3 operates as a critical component in enzymatic systems that protect against oxidative stress. It reduces reactive carbonyls derived from lipid peroxidation products contributing to cellular antioxidant defenses. Although not typically recognized as part of a large complex its activity often synergizes with other reductases and dehydrogenases in these protective roles. This activity helps to limit the damage from lipid peroxidation and therefore maintain cellular function under oxidative stress conditions.

Pathways

CBR3 plays a significant part in the drug metabolism pathway where it metabolizes various therapeutic drugs and environmental toxins. The enzyme interacts closely with NADPH-dependent cofactors and other reductases like CBR1 facilitating phase I detoxification processes. Additionally CBR3 contributes to the prostaglandin synthesis pathway by converting prostaglandin E2 into less active forms interacting relevantly with enzymes involved in prostaglandin metabolism.

CBR3 has been linked to anthracycline-induced cardiotoxicity and certain forms of cancer. In cardiac tissues CBR3 expression influences susceptibility to damage caused by anthracycline antibiotics which are commonly used in cancer chemotherapy. Variations in the gene encoding CBR3 affect the metabolism and clearance of these drugs potentially exacerbating cardiac damage. In cancer CBR3 along with proteins like CBR1 modulates susceptibility to chemotherapeutic agents impacting drug efficacy and toxicity. Understanding the polymorphisms of CBR3 may optimize therapeutic strategies and prevent adverse effects in susceptible individuals.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the NADPH-dependent reduction of carbonyl compounds to their corresponding alcohols (PubMed : 18493841). Has low NADPH-dependent oxidoreductase activity. Acts on several orthoquinones, acts as well on non-quinone compounds, such as isatin or on the anticancer drug oracin (PubMed : 15537833, PubMed : 18493841, PubMed : 19841672). Best substrates for CBR3 is 1,2- naphthoquinone, hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed : 19841672). Exerts activity toward ortho-quinones but not paraquinones. No endogenous substrate for CBR3 except isatin has been identified (PubMed : 19841672).
See full target information CBR3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB156590

Anti-CBR1 antibody [EPR9660]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (AB156590)

  • 0
  • 0
Primary Antibodies

AB134166

Anti-Slit2 antibody [EPR2771]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] (AB134166)

  • 0
  • 0
Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1
Primary Antibodies

AB76149

Anti-YB1 antibody [EP2708Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-YB1 antibody [EP2708Y] (AB76149)

  • 5
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com