AB96252

Anti-CBS antibody

Name: Anti-CBS antibody (ab96252) | Abcam
Brand: Abcam Limited
SKU: AB96252

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(15 Publications)

Rabbit Polyclonal CBS antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Zebrafish, Recombinant fragment samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBS aa 1-250.

View Alternative Names

Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (AB96252)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (AB96252)

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody (AB96252)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody (AB96252)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling CBS protein at cytoplasm and nucleus using ab96252 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).
Scale bar = 10 µM

Supplier Data

Immunoprecipitation - Anti-CBS antibody (AB96252)

Samples were separated by 7.5 % SDS-PAGE. The immunoprecipitated CBS protein was detected by CBS antibody diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-CBS antibody (ab96252) at 1/500 dilution

Lane 1:

HepG2 whole cell extract at 50 µg

Lane 2:

Control with 4 µg of preimmune Rabbit IgG

Lane 3:

HepG2 whole cell extract (+)

Secondary

All lanes:

EasyBlot HRP-conjugated anti rabbit IgG antibody

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-CBS antibody (AB96252)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CBS antibody (ab96252) at 1/3000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

CBS shRNA transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-CBS antibody (AB96252)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CBS antibody (ab96252) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-CBS antibody (AB96252)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CBS antibody (ab96252) at 1/1000 dilution

All lanes:

293T Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 61 kDa

false

Unknown

Western blot - Anti-CBS antibody (AB96252)

All lanes:

Western blot - Anti-CBS antibody (ab96252) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Zebrafish brain homogenate at 20 µg

Lane 3:

Zebrafish heart homogenate at 20 µg

Lane 4:

Zebrafish liver homogenate at 20 µg

Lane 5:

Human epithelial carcinoma cell line at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 61 kDa

Observed band size: 72 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-CBS antibody (AB96252)

All lanes:

Western blot - Anti-CBS antibody (ab96252) at 1/5000 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

CBS Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Zebrafish

Applications

IHC-P, IP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CBS aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P35520

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).

Biological function summary

The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.

Pathways

CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.

CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed : 20506325, PubMed : 23974653, PubMed : 23981774). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).

See full target information CBS

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 79:103450 PubMed39667306

2024

Polysulfide and persulfide-mediated activation of the PERK-eIF2α-ATF4 pathway increases Sestrin2 expression and reduces methylglyoxal toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Koike,Hideo Kimura,Yuki Ogasawara

Cells 13: PubMed38334606

2024

Moderate Elevation of Homocysteine Induces Endothelial Dysfunction through Adaptive UPR Activation and Metabolic Rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Barun Chatterjee,Fabeha Fatima,Surabhi Seth,Soumya Sinha Roy

Experimental animals 73:109-123 PubMed37766548

2023

Middle aged CAMKII-Cre:Cbs mice: a new model for studying perioperative neurocognitive disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Li,Mengfan He,Danqing Dai,Xiaofei Gao,Huazheng Liang,Lize Xiong

Redox biology 59:102595 PubMed36608589

2023

Effects of methionine intake on cognitive function in mild cognitive impairment patients and APP/PS1 Alzheimer's Disease model mice: Role of the cystathionine-β-synthase/HS pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Xi,Yuyu Zhang,Yiwen Zhou,Qing Liu,Xuhui Chen,Xuebo Liu,Tilman Grune,Lin Shi,Min Hou,Zhigang Liu

Neurogastroenterology and motility 35:e14488 PubMed36371703

2022

Dysregulated cystathionine-β-synthase/hydrogen sulfide signaling promotes chronic stress-induced colonic hypermotility in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjuan Lin,Guiying Hu,Baoping Yu

FASEB journal : official publication of the Federa 34:12991-13004 PubMed32777143

2020

Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Colleen M Bartman,Marta Schiliro,Martin Helan,Y S Prakash,David Linden,Christina Pabelick

International journal of molecular medicine 46:782-794 PubMed32468069

2020

Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Tian,Chao-Yang Tan,Qin-Yi Chen,Ying Zhou,Zu-Wei Qu,Meng Zhang,Ke-Tao Ma,Wen-Yan Shi,Li Li,Jun-Qiang Si

Cellular signalling 63:109384 PubMed31394193

2019

O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Guoqing Zhu,Ya Liu,Qi Wu,Xiao Zhang,Zhixuan Bian,Yue Zhang,Qiuhui Pan,Fenyong Sun

Journal of neuroendocrinology 31:e12686 PubMed30633838

2019

Nitric oxide acutely modulates hypothalamic and neurohypophyseal carbon monoxide and hydrogen sulphide production to control vasopressin, oxytocin and atrial natriuretic peptide release in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Coletti,Juliana Bezerra Medeiros de Lima,Fernanda Maria Veanholi Vechiato,Fabiana Lucio de Oliveira,Lucas Kniess Debarba,Gislaine Almeida-Pereira,Lucila Leico Kagohara Elias,José Antunes-Rodrigues

Molecular medicine reports 16:7706-7714 PubMed28944880

2017

Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Hongbo Ci,Guanglei Tian,Yanjun Zhang,Xiaohu Ge

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CBS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (AB96252)

Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody (AB96252)

Immunoprecipitation - Anti-CBS antibody (AB96252)

Western blot - Anti-CBS antibody (AB96252)

Western blot - Anti-CBS antibody (AB96252)

Western blot - Anti-CBS antibody (AB96252)

Western blot - Anti-CBS antibody (AB96252)

Western blot - Anti-CBS antibody (AB96252)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise