Rabbit Polyclonal CBS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Zebrafish, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBS aa 1-250.
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- Redox biology 59:1025952023Effects of methionine intake on cognitive function in mild cognitive impairment patients and APP/PS1 Alzheimer's Disease model mice: Role of the cystathionine-β-synthase/HS pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujia Xi,Yuyu Zhang,Yiwen Zhou,Qing Liu,Xuhui Chen,Xuebo Liu,Tilman Grune,Lin Shi,Min Hou,Zhigang LiuPubMed 36608589
- Neurogastroenterology and motility 35:e144882022Dysregulated cystathionine-β-synthase/hydrogen sulfide signaling promotes chronic stress-induced colonic hypermotility in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjuan Lin,Guiying Hu,Baoping YuPubMed 36371703
- FASEB journal : official publication of the Federa 34:12991-130042020Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColleen M Bartman et. al.PubMed 32777143
- International journal of molecular medicine 46:782-7942020Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Jie Tian et. al.PubMed 32468069
- Cellular signalling 63:1093842019O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Chen et. al.PubMed 31394193
- Journal of neuroendocrinology 31:e126862019Nitric oxide acutely modulates hypothalamic and neurohypophyseal carbon monoxide and hydrogen sulphide production to control vasopressin, oxytocin and atrial natriuretic peptide release in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Coletti,Juliana Bezerra Medeiros de Lima,Fernanda Maria Veanholi Vechiato,Fabiana Lucio de Oliveira,Lucas Kniess Debarba,Gislaine Almeida-Pereira,Lucila Leico Kagohara Elias,José Antunes-RodriguesPubMed 30633838
- Molecular medicine reports 16:7706-77142017Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Song et. al.PubMed 28944880
- International journal of molecular medicine 40:549-5572017Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen‑glucose deprivation‑induced endoplasmic reticulum stress in PC12 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zhang,Yong-Quan Lin,Wei-Sheng Wang,Xin-Qiang WangPubMed 28656194
- Brain pathology (Zurich, Switzerland) 27:449-4582016Upregulation of cystathionine β-synthase and p70S6K/S6 in neonatal hypoxic ischemic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMirna Lechpammer,Yen P Tran,Pia Wintermark,Veronica Martínez-Cerdeño,Viswanathan V Krishnan,Waseem Ahmed,Robert F Berman,Frances E Jensen,Evgeny Nudler,David ZagzagPubMed 27465493
- The British journal of nutrition 112:1274-852014Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarit Espe et. al.PubMed 25196630
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CBS aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P35520
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Zebrafish | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:23981774, PubMed:20506325, PubMed:23974653). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).
Alternative names
Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
Recommended products
Rabbit Polyclonal CBS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Zebrafish, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBS aa 1-250.
Alternative names
Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CBS aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P35520
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).
- Biological function summary
The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.
- Pathways
CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.
- Associated diseases and disorders
CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Western blot - Anti-CBS antibody (ab96252)
All lanes: Western blot - Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: CBS Knockout HeLa cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 61 kDa
Western blot - Anti-CBS antibody (ab96252)
7.5% SDS PAGE
All lanes: Western blot - Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1: Molt-4 whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (ab96252)
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution.
Western blot - Anti-CBS antibody (ab96252)
All lanes: Western blot - Anti-CBS antibody (ab96252) at 1 µg/mL
Lane 1: Marker
Lane 2: Zebrafish brain homogenate at 20 µg
Lane 3: Zebrafish heart homogenate at 20 µg
Lane 4: Zebrafish liver homogenate at 20 µg
Lane 5: Human epithelial carcinoma cell line at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 72 kDa
Exposure time: 3min
Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody (ab96252)
HeLa cells stained for CBS (green) using ab96252 at 1/200 dilution in ICC/IF.
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