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Knockout Tested Rabbit Recombinant Monoclonal CBS antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-CBS antibody [EPR28083-45] (AB313382), expandable thumbnail
  • Western blot - Anti-CBS antibody [EPR28083-45] (AB313382), expandable thumbnail
  • Western blot - Anti-CBS antibody [EPR28083-45] (AB313382), expandable thumbnail
  • Western blot - Anti-CBS antibody [EPR28083-45] (AB313382), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (AB313382), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)IP
Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).

Alternative names

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CBS antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28083-45

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).

Biological function summary

The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.

Pathways

CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.

Associated diseases and disorders

CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR28083-45] (ab313382)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Performed under reducing conditions.
    In Western blot, ab313382 was shown to bind specifically to CBS.
    Target of interest was observed at 61 kDa wild-type Hela cell lysates (lane 1, wild-type cell line Human wild-type HeLa cell line ab255448) with no signal observed at this size in CBS knockout cell line (lane 2, knockout cell line Human CBS knockout HeLa cell line ab264950 / knockout cell lysate Human CBS knockout HeLa cell lysate ab257203).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 26 seconds

    All lanes: Western blot - Anti-CBS antibody [EPR28083-45] (ab313382) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: CBS knock out HeLa whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 61 kDa

    Exposure time: 26s

  • Western blot - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR28083-45] (ab313382)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: skeletal muscle(PMID:25608649).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-CBS antibody [EPR28083-45] (ab313382) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 61 kDa

    Exposure time: 180s

  • Western blot - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR28083-45] (ab313382)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 92 seconds

    All lanes: Western blot - Anti-CBS antibody [EPR28083-45] (ab313382) at 1/1000 dilution

    Lane 1: Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 61 kDa

    Exposure time: 92s

  • Western blot - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR28083-45] (ab313382)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: skeletal muscle(PMID:25608649).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 92 seconds

    All lanes: Western blot - Anti-CBS antibody [EPR28083-45] (ab313382) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat kidney tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 61 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling CBS with ab313382 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat skeletal muscle.The section was incubated with ab313382 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling CBS with ab313382 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse skeletal muscle (PMID: 25608649).The section was incubated with ab313382 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CBS with ab313382 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat liver.The section was incubated with ab313382 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CBS with ab313382 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat kidney.The section was incubated with ab313382 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CBS with ab313382 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse liver (PMID: 25608649).The section was incubated with ab313382 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR28083-45] (ab313382)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CBS with ab313382 at 1/2000 (0.259 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse kidney.The section was incubated with ab313382 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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