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Rabbit Recombinant Monoclonal CBS antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-CBS antibody [EPR8578] (AB131155), expandable thumbnail
  • Western blot - Anti-CBS antibody [EPR8578] (AB131155), expandable thumbnail
  • OI-RD Scanning - Anti-CBS antibody [EPR8578] (AB131155), expandable thumbnail
  • Western blot - Anti-CBS antibody [EPR8578] (AB131155), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:20506325, PubMed:23974653, PubMed:23981774). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).

Alternative names

Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS

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Rabbit Recombinant Monoclonal CBS antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8578
Purification technique
Affinity purification Protein A
Dissociation constant
6.78 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).

Biological function summary

The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.

Pathways

CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.

Associated diseases and disorders

CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CBS antibody [EPR8578] (ab131155), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR8578] (ab131155)

    Lanes 1-3: Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CBS knockout HeLa cell line ab264950 (knockout cell lysate Human CBS knockout HeLa cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CBS knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human CBS knockout HeLa cell line (Human CBS knockout HeLa cell line ab264950)

    Lane 3: Raji cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 61 kDa

    Observed band size: 70 kDa

  • Western blot - Anti-CBS antibody [EPR8578] (ab131155), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR8578] (ab131155)

    Lanes 1-3: Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CBS knockout HeLa cell line ab264950 (knockout cell lysate Human CBS knockout HeLa cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CBS knockout HeLa cell lysate at 20 µg

    Lane 3: Raji cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 61 kDa

    Observed band size: 70 kDa

  • OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155), expandable thumbnail

    OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-CBS antibody [EPR8578] (ab131155), expandable thumbnail

    Western blot - Anti-CBS antibody [EPR8578] (ab131155)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Exposure time: 20s

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Product protocols

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