Rabbit Recombinant Monoclonal CBS antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (AB248359), expandable thumbnail](https://content.abcam.com/products/images/cbs-antibody-epr8578-bsa-and-azide-free-ab248359--western-blot-img3835.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (AB248359)")
![Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (AB248359), expandable thumbnail](https://content.abcam.com/products/images/cbs-antibody-epr8578-bsa-and-azide-free-ab248359--western-blot-img436861.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (AB248359)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (PubMed:20506325, PubMed:23974653, PubMed:23981774). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).
Alternative names
Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS
Recommended products
Rabbit Recombinant Monoclonal CBS antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8578
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 6.78 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248359 is the carrier-free version of Anti-CBS antibody [EPR8578] ab131155.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).
- Biological function summary
The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.
- Pathways
CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.
- Associated diseases and disorders
CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359), expandable thumbnail](https://content.abcam.com/products/images/cbs-antibody-epr8578-bsa-and-azide-free-ab248359--western-blot-img3835.jpg "Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359)")
Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CBS antibody [EPR8578] ab131155).
Lanes 1-3: Merged signal (red and green). Green - Anti-CBS antibody [EPR8578] ab131155 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-CBS antibody [EPR8578] ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CBS knockout HeLa cell line ab264950 (knockout cell lysate Human CBS knockout HeLa cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. Anti-CBS antibody [EPR8578] ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CBS antibody [EPR8578] (Anti-CBS antibody [EPR8578] ab131155) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CBS knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human CBS knockout HeLa cell line (Human CBS knockout HeLa cell line ab264950)
Lane 3: Raji cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa
![Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359), expandable thumbnail](https://content.abcam.com/products/images/cbs-antibody-epr8578-bsa-and-azide-free-ab248359--western-blot-img436861.jpg "Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359)")
Western blot - Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CBS antibody [EPR8578] ab131155)
Blocking and diluting buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-CBS antibody [EPR8578] (Anti-CBS antibody [EPR8578] ab131155) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Exposure time: 20s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com