Anti-CBS antibody [EPR8579]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR8579] (AB140600)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling CBS with ab140600 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody [EPR8579] (AB140600)

Immunofluorescent analysis of MCF7 cells labelling CBS with ab140600 at 1/100 dilution.

• WB

Lab

Western blot - Anti-CBS antibody [EPR8579] (AB140600)

Lanes 1-3 : Merged signal (red and green). Green - ab140600 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab140600 Anti-CBS antibody [EPR8579] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab140600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CBS knockout HeLa cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Predicted band size: 61 kDa

Observed band size: 70 kDa

false

• WB

Lab

Western blot - Anti-CBS antibody [EPR8579] (AB140600)

Lanes 1-3 : Merged signal (red and green). Green - ab140600 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab140600 Anti-CBS antibody [EPR8579] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab140600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CBS knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CBS knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cbs-knockout-hela-cell-line-ab264950'>ab264950</a>)

Lane 3:

Raji cell lysate at 20 µg

Predicted band size: 61 kDa

Observed band size: 70 kDa

false

• WB

Lab

Western blot - Anti-CBS antibody [EPR8579] (AB140600)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 5s