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AB152144

Anti-CBX1 / HP1 beta antibody

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(1 Publication)

Rabbit Polyclonal CBX1 / HP1 beta antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CBX1 aa 1 to C-terminus.

View Alternative Names

CBX, CBX1, Chromobox protein homolog 1, HP1Hsbeta, Heterochromatin protein 1 homolog beta, Heterochromatin protein p25, M31, Modifier 1 protein, p25beta, HP1 beta

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (AB152144)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (AB152144)

Immunohistochemical analysis of paraffin-embedded 59T xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (AB152144)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (AB152144)

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.

Western blot - Anti-CBX1 / HP1 beta antibody (AB152144)
  • WB

Unknown

Western blot - Anti-CBX1 / HP1 beta antibody (AB152144)

12% SDS PAGE

All lanes:

Western blot - Anti-CBX1 / HP1 beta antibody (ab152144) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CBX1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P83916

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CBX1 also known as HP1 beta is a chromatin-associated protein that plays an important role in chromatin organization and epigenetic regulation. This protein has a mass of approximately 22 kDa and localizes within the nucleus where it participates in heterochromatin formation. CBX1 recognizes and binds to histone H3 when it is methylated at lysine 9 (H3K9me3) a modification integral to forming tightly packed DNA regions. Expression of CBX1 occurs widely in many tissue types reflecting its fundamental role in genomic maintenance and cell cycle regulation.
Biological function summary

The CBX1 protein acts as an integral component of the chromatin remodeling complex. By binding to methylated histones CBX1 helps stabilize chromatin architecture and repress transcription of genes within heterochromatic regions. This function is essential for maintaining gene silencing which is critical for processes like development and differentiation. CBX1 collaborates with other HP1 isoforms such as HP1 alpha and HP1 gamma as part of the family of heterochromatin proteins that contribute collectively to chromatin dynamics and gene expression control.

Pathways

CBX1 plays a part in the epigenetic transcriptional repression and cell cycle regulation pathways. It associates with SUV39H1 a methyltransferase to recognize and bind the H3K9me3 mark adding stability to the methylation signal and contributing to gene silencing. Additionally CBX1 interacts with other proteins like DNMT1 a DNA methyltransferase reinforcing its role in sustaining repressive chromatin states and preserving genome integrity during DNA replication.

CBX1 holds a significant association with cancer and neurological disorders. Aberrant expression or mutations in CBX1 can lead to disrupted chromatin states influencing oncogene activation or tumor suppressor gene silencing which are common events in cancer development. Furthermore altered CBX1 function links to neurological disorders where inappropriate chromatin remodeling contributes to disease pathology. Changes in the expression and interaction with other proteins including HP1 alpha highlight its potential role in disease mechanisms making CBX1 a relevant target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
See full target information CBX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biological research 58:59 PubMed40877912

2025

The unique contributions of Rab11 and Rab35 to the completion of cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Paulius Gibieža,Emilija Ratkevičiūtė,Girstautė Dabkevičiūtė,Vilma Petrikaitė
View all publications
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Product promise

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