Rabbit Recombinant Monoclonal CBX2 antibody. Suitable for WB and reacts with Human, Rat samples. Immunogen corresponding to Synthetic Peptide within Human CBX2.
Images
![Western blot - Anti-CBX2 antibody [24A2] (AB314187), expandable thumbnail](https://content.abcam.com/products/images/cbx2-antibody-24a2-ab314187--western-blot-img419524.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CBX2 antibody [24A2] (AB314187)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human CBX2. Database link Q14781
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species Rat | Dilution info 1/500 - 1/2000 | Notes - |
Target data
Function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity). Involved in sexual development, acting as activator of NR5A1 expression (PubMed:19361780).
Alternative names
Chromobox protein homolog 2, CBX2
Recommended products
Rabbit Recombinant Monoclonal CBX2 antibody. Suitable for WB and reacts with Human, Rat samples. Immunogen corresponding to Synthetic Peptide within Human CBX2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human CBX2. Database link Q14781
- Clone number
- 24A2
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 'CBX2' protein also known as chromobox homolog 2 is an important component of the genetic regulation machinery. It weighs approximately 64 kDa and is mainly expressed in the nucleus of cells. CBX2 belongs to the Polycomb group (PcG) proteins specifically forming a part of the Polycomb repressive complex 1 (PRC1). Through its interaction with chromatin CBX2 induces transcriptional repression an important function for maintaining cellular identity and pluripotency.
- Biological function summary
The effects of this protein extend to important roles in embryogenesis and cell cycle regulation. CBX2 serves as a part of PRC1 where it acts to mark chromatin for activation or repression. This complex allows CBX2 to recruit additional enzymes and proteins that modify chromatin structure ensuring tight regulation of gene expression. Cell differentiation depends heavily on the epigenetic control mediated by such proteins highlighting the importance of CBX2 in developmental biology.
- Pathways
CBX2 plays integral roles in both the Wnt and the Hedgehog signaling pathways impacting cellular growth and differentiation. In these pathways CBX2 interacts with proteins like β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway influencing gene transcription. These interactions highlight CBX2’s importance in switching genetic programs on or off in response to cellular signaling.
- Associated diseases and disorders
Researchers have associated CBX2 with cancer and sexual development disorders. Abnormal expression patterns of CBX2 are often found in various cancer types where it can interact with proteins like BMI1 to alter chromatin states. Additionally mutations in CBX2 have been linked to disorders of sex development (DSD) where it influences genes critical for sexual differentiation. Understanding CBX2’s role could cast light on pathologies related to these diseases and provide potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-CBX2 antibody [24A2] (ab314187), expandable thumbnail](https://content.abcam.com/products/images/cbx2-antibody-24a2-ab314187--western-blot-img419524.jpg "Western blot - Anti-CBX2 antibody [24A2] (ab314187)")
Western blot - Anti-CBX2 antibody [24A2] (ab314187)
Western Blot
Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, Rat heart tissue
All lanes: CBX2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57, 24 kDa
Observed band size: 60-72 kDaAll lanes: Western blot - Anti-CBX2 antibody [24A2] (ab314187) at 1/2000 dilution
Lane 1: U251 MG whole cell lysate
Lane 2: Ntera-2 whole cell lysate
Lane 3: HeLa whole cell lysate
Lane 4: MCF7 whole cell lysate
Lane 5: HepG2 whole cell lysate
Lane 6: HEK293 whole cell lysate
Lane 7: Rat heart tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60-72 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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