Rabbit Polyclonal CBX2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBX2 aa 1-250.
Images
Publications
Filter by
- Experimental and therapeutic medicine 26:5622023miR‑149‑3p suppresses the proliferation and metastasis of glioma cells by targeting the CBX2/Wnt/β‑catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanjun Wang,Yanqun Song,Zhongcheng Liu,Junmin Li,Guodong Wang,Hong Pan,Zhiming ZhengPubMed 37954123
- American journal of translational research 14:1188-12032022Cannabidiol promotes apoptosis of osteosarcoma cells and by activating the SP1-CBX2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangxing Xu,Guiyuan Sun,Zhibin Peng,Jingsong Liu,Zecheng Li,Jinglong YanPubMed 35273722
- Technology in cancer research & treatment 20:153303382110458312021CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLe Wang,Bingcheng Ren,Hao Zhuang,Yue Zhong,Yang NanPubMed 34709960
- Molecular medicine reports 23:2020CBX2 depletion inhibits the proliferation, invasion and migration of gastric cancer cells by inactivating the YAP/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiaomiao Zeng,Bangxue Li,Lei Yang,Quanlin GuanPubMed 33313949
- Journal of Cancer 11:5198-52092020The Prognostic Value of the Chromobox Family in Human Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYichi Xu et. al.PubMed 32742466
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CBX2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q14781
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity). Involved in sexual development, acting as activator of NR5A1 expression (PubMed:19361780).
Alternative names
Chromobox protein homolog 2, CBX2
Recommended products
Rabbit Polyclonal CBX2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBX2 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CBX2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q14781
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 'CBX2' protein also known as chromobox homolog 2 is an important component of the genetic regulation machinery. It weighs approximately 64 kDa and is mainly expressed in the nucleus of cells. CBX2 belongs to the Polycomb group (PcG) proteins specifically forming a part of the Polycomb repressive complex 1 (PRC1). Through its interaction with chromatin CBX2 induces transcriptional repression an important function for maintaining cellular identity and pluripotency.
- Biological function summary
The effects of this protein extend to important roles in embryogenesis and cell cycle regulation. CBX2 serves as a part of PRC1 where it acts to mark chromatin for activation or repression. This complex allows CBX2 to recruit additional enzymes and proteins that modify chromatin structure ensuring tight regulation of gene expression. Cell differentiation depends heavily on the epigenetic control mediated by such proteins highlighting the importance of CBX2 in developmental biology.
- Pathways
CBX2 plays integral roles in both the Wnt and the Hedgehog signaling pathways impacting cellular growth and differentiation. In these pathways CBX2 interacts with proteins like β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway influencing gene transcription. These interactions highlight CBX2’s importance in switching genetic programs on or off in response to cellular signaling.
- Associated diseases and disorders
Researchers have associated CBX2 with cancer and sexual development disorders. Abnormal expression patterns of CBX2 are often found in various cancer types where it can interact with proteins like BMI1 to alter chromatin states. Additionally mutations in CBX2 have been linked to disorders of sex development (DSD) where it influences genes critical for sexual differentiation. Understanding CBX2’s role could cast light on pathologies related to these diseases and provide potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-CBX2 antibody (ab235305)
All lanes: Western blot - Anti-CBX2 antibody (ab235305) at 1/500 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2: Mouse heart tissue lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (ab235305)
Paraffin-embedded human breast cancer tissue stained for CBX2 using ab235305 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (ab235305)
Paraffin-embedded human tonsil tissue stained for CBX2 using ab235305 at 1/100 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com