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AB235305

Anti-CBX2 antibody

3

(1 Review)

|

(8 Publications)

Rabbit Polyclonal CBX2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CBX2 aa 1-250.

View Alternative Names

Chromobox protein homolog 2, CBX2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

Paraffin-embedded human tonsil tissue stained for CBX2 using ab235305 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX2 antibody (AB235305)

Paraffin-embedded human breast cancer tissue stained for CBX2 using ab235305 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CBX2 antibody (AB235305)
  • WB

Supplier Data

Western blot - Anti-CBX2 antibody (AB235305)

All lanes:

Western blot - Anti-CBX2 antibody (ab235305) at 1/500 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 2:

Mouse heart tissue lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CBX2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q14781

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'CBX2' protein also known as chromobox homolog 2 is an important component of the genetic regulation machinery. It weighs approximately 64 kDa and is mainly expressed in the nucleus of cells. CBX2 belongs to the Polycomb group (PcG) proteins specifically forming a part of the Polycomb repressive complex 1 (PRC1). Through its interaction with chromatin CBX2 induces transcriptional repression an important function for maintaining cellular identity and pluripotency.
Biological function summary

The effects of this protein extend to important roles in embryogenesis and cell cycle regulation. CBX2 serves as a part of PRC1 where it acts to mark chromatin for activation or repression. This complex allows CBX2 to recruit additional enzymes and proteins that modify chromatin structure ensuring tight regulation of gene expression. Cell differentiation depends heavily on the epigenetic control mediated by such proteins highlighting the importance of CBX2 in developmental biology.

Pathways

CBX2 plays integral roles in both the Wnt and the Hedgehog signaling pathways impacting cellular growth and differentiation. In these pathways CBX2 interacts with proteins like β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway influencing gene transcription. These interactions highlight CBX2's importance in switching genetic programs on or off in response to cellular signaling.

Researchers have associated CBX2 with cancer and sexual development disorders. Abnormal expression patterns of CBX2 are often found in various cancer types where it can interact with proteins like BMI1 to alter chromatin states. Additionally mutations in CBX2 have been linked to disorders of sex development (DSD) where it influences genes critical for sexual differentiation. Understanding CBX2's role could cast light on pathologies related to these diseases and provide potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity). Involved in sexual development, acting as activator of NR5A1 expression (PubMed : 19361780).
See full target information CBX2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1430891 PubMed39114365

2024

CBX2 enhances the progression and TMZ chemoresistance of glioma via EZH2-mediated epigenetic silencing of PTEN expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Bo Yang,Yingzhao Wang,Shuhan Liu,Changkai Ma,Jianmin Piao,Shiqiang Ma,Dehai Yu,Wei Wu

Experimental and therapeutic medicine 26:562 PubMed37954123

2023

miR‑149‑3p suppresses the proliferation and metastasis of glioma cells by targeting the CBX2/Wnt/β‑catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Wang,Yanqun Song,Zhongcheng Liu,Junmin Li,Guodong Wang,Hong Pan,Zhiming Zheng

BMC cancer 23:641 PubMed37430195

2023

An integrative bioinformatics investigation and experimental validation of chromobox family in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fenling Zhou,Lu Chen,Peng Lu,Yuli Cao,Cuilan Deng,Gexiu Liu

American journal of cancer research 13:1363-1376 PubMed37168348

2023

EBNA2 mediates lipid metabolism and tumorigenesis through activation of ATF4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Feng,Ping Zhang,Paul Yao,Hongyu Zhang

American journal of translational research 14:1188-1203 PubMed35273722

2022

Cannabidiol promotes apoptosis of osteosarcoma cells and by activating the SP1-CBX2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangxing Xu,Guiyuan Sun,Zhibin Peng,Jingsong Liu,Zecheng Li,Jinglong Yan

Technology in cancer research & treatment 20:15330338211045831 PubMed34709960

2021

CBX2 Induces Glioma Cell Proliferation and Invasion Through the Akt/PI3K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Le Wang,Bingcheng Ren,Hao Zhuang,Yue Zhong,Yang Nan

Molecular medicine reports 23: PubMed33313949

2020

CBX2 depletion inhibits the proliferation, invasion and migration of gastric cancer cells by inactivating the YAP/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Zeng,Bangxue Li,Lei Yang,Quanlin Guan

Journal of Cancer 11:5198-5209 PubMed32742466

2020

The Prognostic Value of the Chromobox Family in Human Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yichi Xu,Shuya Pan,Yizuo Song,Chunyu Pan,Cheng Chen,Xueqiong Zhu
View all publications
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