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AB229256

Anti-Cbx6 antibody

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(1 Publication)

Rabbit Polyclonal CBX6 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CBX6.

View Alternative Names

Chromobox protein homolog 6, CBX6

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cbx6 antibody (AB229256)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cbx6 antibody (AB229256)

A549 (human lung carcinoma cell line) cells stained for Cbx6 (green) using ab229256 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CBX6. The exact immunogen used to generate this antibody is proprietary information.

O95503

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cbx6 also known as Chromobox Protein Homolog 6 is a component of the Polycomb Group (PcG) proteins with a core function in transcriptional repression. It has a molecular mass of approximately 51 kDa. The primary expression of Cbx6 occurs in stem and progenitor cells but it is also present in various tissues during embryogenesis. The protein contains a chromodomain that facilitates its interaction with histones and contributes to chromatin structure alteration impacting gene expression regulation.
Biological function summary

Cbx6 plays a significant role in maintaining cellular pluripotency and regulating differentiation processes. As part of the Polycomb Repressive Complex 1 (PRC1) Cbx6 participates in modifying chromatin to silence target genes. This complex works synergistically with other PcG proteins to ensure correct temporal and spatial expression of developmentally important genes. The association with histone H3 lysine 27 trimethylation (H3K27me3) is fundamental to its repressive function.

Pathways

Cbx6 functions integrally within the epigenetic regulation pathway and impacts the Wnt signaling pathway. By interacting with other PRC1 members Cbx6 facilitates transcriptional silencing required for the maintenance of stem cell identity. Its activities are closely linked to the modulation of canonical Wnt signaling and in doing so it affects proteins such as β-catenin as well as interacting with other PcG components like Cbx7 and Bmi1.

Cbx6 shows associations with various cancers and developmental disorders. Its dysregulation is observed in malignancies like colorectal cancer where abnormal Cbx6 expression affects the Wnt signaling pathway leading to enhanced proliferation. Furthermore disorders in embryonic development can arise from improper Cbx6 function in Polycomb-mediated gene silencing. Within the context of these conditions Cbx6 works alongside other regulators such as EZH2 in the PRC2 complex highlighting its multifaceted role in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin (PubMed : 18927235). Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).
See full target information CBX6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed32352376

2020

DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Wang,Zhiming Chen,Marcel Mettlen,Jungsik Noh,Sandra L Schmid,Gaudenz Danuser
View all publications
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