AB91431

Anti-cbx7 antibody

Name: Anti-cbx7 antibody (ab91431) | Abcam
Brand: Abcam Limited
SKU: AB91431
Price: 545 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal CBX7 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Chromobox protein homolog 7 aa 200 to C-terminus.

View Alternative Names

Chromobox protein homolog 7, CBX7

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody (AB91431)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody (AB91431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling cbx7 with ab91431 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-cbx7 antibody (AB91431)

cbx7 was immunoprecipitated from HeLa whole cell lysate, using ab91431 at 10 μg/mg of lysate. 20% of the immunoprecipitae was loaded per lane for the subsequent Western blot and probed with ab91431 at 1 μg/ml (lane 1) or a control IgG (lane 2).
Detetion : chemoluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-cbx7 antibody (ab91431)

Predicted band size: 28 kDa

false

Unknown

Western blot - Anti-cbx7 antibody (AB91431)

All lanes:

Western blot - Anti-cbx7 antibody (ab91431) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 28 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human Chromobox protein homolog 7 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95931

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to cbx7 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CBX7 also known as Chromobox protein homolog 7 is a member of the Polycomb group proteins. It has a molecular mass of approximately 27 kDa. Mechanically CBX7 functions as a transcriptional repressor that interacts with chromatin to maintain the repression of genes involved in development and cell differentiation. The protein is expressed in various tissues with notable levels in embryonic stem cells and certain adult tissues like bone marrow and the immune system.

Biological function summary

The CBX7 protein participates in the complex known as Polycomb Repressive Complex 1 (PRC1). This complex plays a significant role in the maintenance of cellular identity by regulating gene expression profiles important for stem cell pluripotency and differentiation. CBX7 binds to modified histones leading to chromatin compaction and gene silencing instrumental for preserving cell lineage integrity during development and cellular transformation.

Pathways

CBX7 interacts with several components in the cell cycle regulation and epigenetic modification pathways. Within the cell cycle regulation pathway it closely interacts with other Polycomb group proteins to control gene expression that governs cell proliferation and differentiation. In the context of epigenetic pathways CBX7 influences chromatin remodeling by interacting with proteins like BMI1 and RING1 thereby exerting control over the transcriptional landscape required for developmental and adaptive responses.

CBX7 shows a relationship with cancer and developmental disorders. Aberrant expression or mutation of CBX7 has been associated with several cancers including prostate and gastric cancers often connected with the suppression of tumor suppressor genes and cell cycle regulation. In these diseases CBX7 interacts with proteins like E2F6 and MYC which are important for cellular proliferation and growth. Moreover disruptions in CBX7 expression patterns can lead to improper cell differentiation contributing to developmental abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Promotes histone H3 trimethylation at 'Lys-9' (H3K9me3). Binds to trimethylated lysine residues in histones, and possibly also other proteins. Regulator of cellular lifespan by maintaining the repression of CDKN2A, but not by inducing telomerase activity.

See full target information Chromobox protein homolog 7

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e14655 PubMed37025896

2023

The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobei Zhang,Meng Jin,Shiqi Liu,Mingde Zang,Lei Hu,Tao Du,Baogui Zhang

Translational oncology 24:101468 PubMed35843065

2022

CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Dai,Yiqi Liu,Renxian Cao,Jingying Cao

American journal of cancer research 12:1934-1959 PubMed35693076

2022

Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Gao,Liqiang Wang,Changfeng Li

Molecular therapy oncolytics 15:108-116 PubMed31709304

2019

CBX7 Inhibits Cell Growth and Motility and Induces Apoptosis in Cervical Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Qi Yan,Ping Tian,Yan Wang,Jing Wang,Ning Tao,Li Ning,Xin Lin,Lu Ding,Jiwen Liu,Cailing Ma

Molecular biology of the cell 28:1208-1222 PubMed28298486

2017

Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Mizuno,Roger D Sloboda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-cbx7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody (AB91431)

Immunoprecipitation - Anti-cbx7 antibody (AB91431)

Western blot - Anti-cbx7 antibody (AB91431)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise