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AB279708

Anti-Cbx8 antibody [EPR23800-14] - BSA and Azide free

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Rabbit Recombinant Monoclonal CBX8 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

PC3, RC1, CBX8, Chromobox protein homolog 8, Polycomb 3 homolog, Rectachrome 1, Pc3, hPc3

1 Images
Western blot - Anti-Cbx8 antibody [EPR23800-14] - BSA and Azide free (AB279708)
  • WB

Lab

Western blot - Anti-Cbx8 antibody [EPR23800-14] - BSA and Azide free (AB279708)

This data was developed using ab259849, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Incubation for primary antibody was carried out overnight at 4 degree.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-Cbx8 antibody [EPR23800-14] (<a href='/en-us/products/primary-antibodies/cbx8-antibody-epr23800-14-ab259849'>ab259849</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 3:

Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa,48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23800-14

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab279708 is the carrier-free version of ab259849.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cbx8 also known as Chromobox protein homolog 8 is part of the Polycomb group proteins with a molecular mass of approximately 50 kDa. Cbx8 functions as a transcriptional regulator by binding to histones and repressive chromatin states. It has a broad expression including in embryonic stem cells and various adult tissues. Its involvement extends to chromatin modification and impacts gene expression regulation making it significant in controlling cellular identity and differentiation.
Biological function summary

Cbx8 contributes to the regulation of gene silencing within the Polycomb Repressive Complex 1 (PRC1). It plays an important role in maintaining cellular memory by ensuring heritable transcriptional repression of target genes. Cbx8's involvement in histone modification particularly histone H3 lysine 27 trimethylation (H3K27me3) is a part of its mechanism to regulate gene expression. This regulation affects key developmental processes and signals cellular differentiation pathways critical for tissue development and maintenance.

Pathways

Cbx8 engages in the Polycomb pathway where it interacts with other PRC1 proteins playing a role in chromatin compaction and silencing of developmental genes. It associates with pathways involved in cell cycle regulation and differentiation linking to essential proteins like RING1 and BMI1. These interactions enable Cbx8 to influence the transcriptional repression necessary for proper execution of developmental programs and cellular response to external signals.

Cbx8 has affiliations with cancer and developmental disorders due to its role in gene silencing. Abnormal Cbx8 activity may lead to disruptions in normal cell cycle controls contributing to oncogenesis with other PRC1 members like BMI1. It also connects to certain developmental syndromes where polycomb dysfunction impacts normal tissue development indicating its broader significance in maintaining cellular homeostasis and genomic stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
See full target information CBX8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com