AB68302

Anti-CC2D1A antibody

Name: Anti-CC2D1A antibody (ab68302) | Abcam
Brand: Abcam Limited
SKU: AB68302

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(4 Publications)

Mouse Polyclonal CC2D1A antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CC2D1A.

View Alternative Names

AKI1, LGD2, CC2D1A, Coiled-coil and C2 domain-containing protein 1A, Akt kinase-interacting protein 1, Five prime repressor element under dual repression-binding protein 1, Putative NF-kappa-B-activating protein 023N, FRE under dual repression-binding protein 1, Freud-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody (AB68302)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody (AB68302)

Immunofluorescence of ab68302 staining on HeLa cell at a concentration of 10 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CC2D1A antibody (AB68302)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CC2D1A antibody (AB68302)

Immunoperoxidase of ab68302 on formalin-fixed paraffin-embedded human small intestine. Antibody concentration, 3 ug/ml.

Unknown

Western blot - Anti-CC2D1A antibody (AB68302)

All lanes:

Western blot - Anti-CC2D1A antibody (ab68302) at 1/500 dilution

All lanes:

human liver tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 104 kDa

Observed band size: 125 kDa

false

Unknown

Western blot - Anti-CC2D1A antibody (AB68302)

All lanes:

Western blot - Anti-CC2D1A antibody (ab68302) at 1/500 dilution

Lane 1:

CC2D1A transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 104 kDa

Observed band size: 104 kDa,125 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CC2D1A.

Q6P1N0

Specificity

This antibody is reactive against mammalian transfected lysate.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CC2D1A also known as Coiled-Coil and C2 Domain Containing 1A is a protein of approximately 100 kDa. This protein is widely expressed with significant presence in the brain indicating its potential role in neural functions. It possesses domains that enable interactions with other proteins and membranes suggesting a multifunctional mechanical role in cellular processes.

Biological function summary

CC2D1A participates in regulating signal transduction and intracellular communication. It becomes part of a larger signaling complex involved in essential cellular processes including proliferation and migration. In neural cells it influences synaptic signaling pathways impacting learning and memory formation suggesting its significance in cognitive functions.

Pathways

CC2D1A participates in the NF-kB and AKT/mTOR signaling pathways which are vital for regulating cell survival and growth. Through these pathways CC2D1A interacts with proteins such as AKT1 and IKK modulating cellular responses to stress and growth factors. Its role in these pathways places it at an important intersection of survival and developmental signaling.

CC2D1A is linked to mental health conditions including autism spectrum disorders (ASD) and intellectual disabilities. Mutations in CC2D1A disrupt its function in neural pathways which may contribute to the development of these disorders. Additionally it interacts with protein DISC1 which has also been implicated in psychiatric disorders highlighting the complex network of interactions in neurodevelopmental and psychiatric disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. May play a role in the altered regulation of HTR1A associated with anxiety and major depression. Mediates HDAC-independent repression of HTR1A promoter in neuronal cell. Performs essential function in controlling functional maturation of synapses (By similarity). Plays distinct roles depending on its localization. When cytoplasmic, acts as a scaffold protein in the PI3K/PDK1/AKT pathway. Repressor of HTR1A when nuclear. In the centrosome, regulates spindle pole localization of the cohesin subunit SCC1/RAD21, thereby mediating centriole cohesion during mitosis.

See full target information CC2D1A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:881571 PubMed35592111

2022

Contributes to the Regulation of Developmental Myelination in the Central Nervous System.

Applications

Unspecified application

Species

Unspecified reactive species

Jenica Acheta,Jiayue Hong,Haley Jeanette,Simrandeep Brar,Anish Yalamanchili,M Laura Feltri,M Chiara Manzini,Sophie Belin,Yannick Poitelon

Frontiers in molecular neuroscience 12:177 PubMed31379499

2019

YAP and TAZ Regulate and in Schwann Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Belin Sophie,Herron Jacob,VerPlank J S Jordan,Park Yungki,Feltri M Laura,Poitelon Yannick

Journal of proteome research 13:4074-90 PubMed25093993

2014

Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6.

Applications

Unspecified application

Species

Unspecified reactive species

Edmarcia Elisa de Souza,Gabriela Vaz Meirelles,Bárbara Biatriz Godoy,Arina Marina Perez,Juliana Helena Costa Smetana,Stephen J Doxsey,Mark E McComb,Catherine E Costello,Stephen A Whelan,Jörg Kobarg

Cell reports 8:647-55 PubMed25066123

2014

CC2D1A regulates human intellectual and social function as well as NF-κB signaling homeostasis.

Applications

Species

Human

M Chiara Manzini,Lan Xiong,Ranad Shaheen,Dimira E Tambunan,Stefania Di Costanzo,Vanessa Mitisalis,David J Tischfield,Antonella Cinquino,Mohammed Ghaziuddin,Mehtab Christian,Qin Jiang,Sandra Laurent,Zohair A Nanjiani,Saima Rasheed,R Sean Hill,Sofia B Lizarraga,Danielle Gleason,Diya Sabbagh,Mustafa A Salih,Fowzan S Alkuraya,Christopher A Walsh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CC2D1A antibody

Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody (AB68302)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CC2D1A antibody (AB68302)

Western blot - Anti-CC2D1A antibody (AB68302)

Western blot - Anti-CC2D1A antibody (AB68302)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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