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Rabbit Recombinant Monoclonal CC2D1A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472), expandable thumbnail
  • Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472), expandable thumbnail
  • Western blot - Anti-CC2D1A antibody [EPR18421] (AB191472), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (AB191472), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CC2D1A antibody [EPR18421] (AB191472), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

-

Species
Rat
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. May play a role in the altered regulation of HTR1A associated with anxiety and major depression. Mediates HDAC-independent repression of HTR1A promoter in neuronal cell. Performs essential function in controlling functional maturation of synapses (By similarity). Plays distinct roles depending on its localization. When cytoplasmic, acts as a scaffold protein in the PI3K/PDK1/AKT pathway. Repressor of HTR1A when nuclear. In the centrosome, regulates spindle pole localization of the cohesin subunit SCC1/RAD21, thereby mediating centriole cohesion during mitosis.

Alternative names

AKI1, LGD2, CC2D1A, Coiled-coil and C2 domain-containing protein 1A, Akt kinase-interacting protein 1, Five prime repressor element under dual repression-binding protein 1, Putative NF-kappa-B-activating protein 023N, FRE under dual repression-binding protein 1, Freud-1

Recommended products

Rabbit Recombinant Monoclonal CC2D1A antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18421
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CC2D1A also known as Coiled-Coil and C2 Domain Containing 1A is a protein of approximately 100 kDa. This protein is widely expressed with significant presence in the brain indicating its potential role in neural functions. It possesses domains that enable interactions with other proteins and membranes suggesting a multifunctional mechanical role in cellular processes.

Biological function summary

CC2D1A participates in regulating signal transduction and intracellular communication. It becomes part of a larger signaling complex involved in essential cellular processes including proliferation and migration. In neural cells it influences synaptic signaling pathways impacting learning and memory formation suggesting its significance in cognitive functions.

Pathways

CC2D1A participates in the NF-kB and AKT/mTOR signaling pathways which are vital for regulating cell survival and growth. Through these pathways CC2D1A interacts with proteins such as AKT1 and IKK modulating cellular responses to stress and growth factors. Its role in these pathways places it at an important intersection of survival and developmental signaling.

Associated diseases and disorders

CC2D1A is linked to mental health conditions including autism spectrum disorders (ASD) and intellectual disabilities. Mutations in CC2D1A disrupt its function in neural pathways which may contribute to the development of these disorders. Additionally it interacts with protein DISC1 which has also been implicated in psychiatric disorders highlighting the complex network of interactions in neurodevelopmental and psychiatric disease pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472), expandable thumbnail

    Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution

    Lane 1: Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 104 kDa

    Observed band size: 130 kDa

    Exposure time: 5s

  • Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472), expandable thumbnail

    Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)

    5% NFDM/TBST: Blocking and diluting buffer.

    The band at 67KD is an isoform of CC2D1A.

    Rogaeva A and Albert PR. Eur J Neurosci. 2007; 26(4):965-974.

    All lanes: Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/5000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 3: PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 104 kDa

    Observed band size: 130 kDa, 67 kDa

    Exposure time: 5s

  • Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472), expandable thumbnail

    Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472)

    5% NFDM/TBST: Blocking and diluting buffer.

    All lanes: Western blot - Anti-CC2D1A antibody [EPR18421] (ab191472) at 1/20000 dilution

    All lanes: Mouse brain tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 104 kDa

    Observed band size: 130 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (ab191472), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CC2D1A antibody [EPR18421] (ab191472)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab191472 at 1/150 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-CC2D1A antibody [EPR18421] (ab191472), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CC2D1A antibody [EPR18421] (ab191472)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CC2D1A with ab191472 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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