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AB276065

Anti-CCDC134 antibody [026] - BSA and Azide free

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Rabbit Recombinant Monoclonal CCDC134 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 134 aa 1 to C-terminus.

View Alternative Names

Coiled-coil domain-containing protein 134, CCDC134

2 Images
Immunoprecipitation - Anti-CCDC134 antibody [026] - BSA and Azide free (AB276065)
  • IP

Unknown

Immunoprecipitation - Anti-CCDC134 antibody [026] - BSA and Azide free (AB276065)

CCDC134 was immunoprecipitated from 0.5 mg HepG2 whole cell lysate using ab276065 at 1/200 dilution, followed by a Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution. Developed using the odssey technique. Performed under reducing conditions.

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Immunoprecipitation - Anti-CCDC134 antibody [026] - BSA and Azide free (ab276065)

Predicted band size: 26 kDa

false

Western blot - Anti-CCDC134 antibody [026] - BSA and Azide free (AB276065)
  • WB

Unknown

Western blot - Anti-CCDC134 antibody [026] - BSA and Azide free (AB276065)

All lanes:

Western blot - Anti-CCDC134 antibody [026] - BSA and Azide free (ab276065) at 1/500 dilution

All lanes:

HL-60 whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

026

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 134 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H6E4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed : 25125657). Enhances cytotoxic anti-tumor activity of CD8(+) T cells (PubMed : 25125657). May inhibit ERK and JNK signaling activity (PubMed : 18087676, PubMed : 23070808). May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling (PubMed : 23070808). Has a critical role in the regulation of osteogenesis and bone development (PubMed : 32181939).. In the nucleus, enhances stability of the PCAF histone acetyltransferase (HAT) complex member TADA2A and thus promotes PCAF-mediated H3K14 and H4K8 HAT activity. May inhibit TADA2A-mediated TP53/p53 'Lys-321' acetylation, leading to reduced TP53 stability and transcriptional activity. May also promote TADA2A-mediated XRCC6 acetylation thus facilitating cell apoptosis in response to DNA damage.
See full target information Coiled-coil domain-containing protein 134
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