Rabbit Polyclonal CCDC40 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCDC40 aa 1-150.
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Publications
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- Life science alliance 1:e2018000702018Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannelore Hartmann et. al.PubMed 30456350
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CCDC40 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q4G0X9
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella (PubMed:21131974). Probably acts together with CCDC39 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella (By similarity). Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39 (PubMed:21131974).
Alternative names
KIAA1640, CCDC40, Coiled-coil domain-containing protein 40
Recommended products
Rabbit Polyclonal CCDC40 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCDC40 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CCDC40 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q4G0X9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC40 antibody (ab121727)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton (microtubules). Recommended concentration of ab121727 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC40 antibody (ab121727)
ab121727 at 1/500 dilution staining CCDC40 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
Western blot - Anti-CCDC40 antibody (ab121727)
All lanes: Western blot - Anti-CCDC40 antibody (ab121727) at 1/250 dilution
Lane 1: RT4 cell lysate
Lane 2: U251 MG cell lysate
Lane 3: Human Plasma
Lane 4: Human Liver tissue lysate
Lane 5: Human Tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 130 kDa
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