Rabbit Polyclonal CCDC47 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCDC47 aa 400 to C-terminus.
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Publications
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- Nature 614:160-1672023Visualization of translation and protein biogenesis at the ER membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMax Gemmer,Marten L Chaillet,Joyce van Loenhout,Rodrigo Cuevas Arenas,Dimitrios Vismpas,Mariska Gröllers-Mulderij,Fujiet A Koh,Pascal Albanese,Richard A Scheltema,Stuart C Howes,Abhay Kotecha,Juliette Fedry,Friedrich FörsterPubMed 36697828
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CCDC47 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96A33
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Xenopus laevis, Zebrafish, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 - 1/1000 | Notes - |
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Xenopus laevis, Zebrafish, Orangutan | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32814900, PubMed:32820719, PubMed:36261522). The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed:36261522). Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein (By similarity). Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex (By similarity). Involved in the regulation of calcium ion homeostasis in the ER (PubMed:30401460). Required for proper protein degradation via the ERAD (ER-associated degradation) pathway (PubMed:25009997). Has an essential role in the maintenance of ER organization during embryogenesis (By similarity).
Alternative names
GK001, MSTP041, PSEC0077, CCDC47, PAT complex subunit CCDC47, Calumin, Coiled-coil domain-containing protein 47
Recommended products
Rabbit Polyclonal CCDC47 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCDC47 aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CCDC47 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96A33
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to CCDC47 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-CCDC47 antibody (ab241608)
Prepared using NETN lysis buffer.
All lanes: Western blot - Anti-CCDC47 antibody (ab241608) at 0.1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-239T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 56 kDa
Exposure time: 10s
Immunoprecipitation - Anti-CCDC47 antibody (ab241608)
CCDC47 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241608 used for IP at 6 μg per reaction. For WB 0.4 μg/ml.Lane 1: ab241608 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Chemiluminescence detection: 10 seconds.
All lanes: Immunoprecipitation - Anti-CCDC47 antibody (ab241608)
Predicted band size: 56 kDa
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