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AB127169

Anti-CCDC50 antibody

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(4 Publications)

Rabbit Polyclonal CCDC50 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC50 aa 1-150.

View Alternative Names

C3orf6, CCDC50, Coiled-coil domain-containing protein 50, Protein Ymer

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC50 antibody (AB127169)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC50 antibody (AB127169)

ab127169, at 1/500 dilution, analysis of paraffin-embedded Hepatoma by immunohistochemical

Immunocytochemistry/ Immunofluorescence - Anti-CCDC50 antibody (AB127169)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CCDC50 antibody (AB127169)

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ab127169 (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1/2000.

Western blot - Anti-CCDC50 antibody (AB127169)
  • WB

Unknown

Western blot - Anti-CCDC50 antibody (AB127169)

10% SDS PAGE

All lanes:

Western blot - Anti-CCDC50 antibody (ab127169) at 1/5000 dilution

Lane 1:

293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CCDC50 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8IVM0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in EGFR signaling.
See full target information CCDC50
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 7: PubMed38803224

2024

K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Waltho,Oliver Popp,Christopher Lenz,Lukas Pluska,Mahil Lambert,Volker Dötsch,Philipp Mertins,Thomas Sommer

Frontiers in genetics 13:975542 PubMed36147484

2022

Single-cell RNA sequencing analysis to explore immune cell heterogeneity and novel biomarkers for the prognosis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Xu,Yao Wang,Leilei Liang,Nan Song

Journal of experimental & clinical cancer research 39:116 PubMed32560659

2020

HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395.

Applications

Unspecified application

Species

Unspecified reactive species

Guoliang Sun,Hui Zhou,Ke Chen,Jin Zeng,Yangjun Zhang,Libin Yan,Weimin Yao,Junhui Hu,Tao Wang,Jinchun Xing,Kefeng Xiao,Lily Wu,Zhangqun Ye,Hua Xu

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 36:9411-9 PubMed26113408

2015

High expression of CD39/ENTPD1 in malignant epithelial cells of human rectal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Bo Cheng,Feng-Sheng Li,Jian-Hua Ding,Ying-Ying Feng,Guang-Zuan Zhuo,Hua-Feng Wei,Ke Zhao
View all publications
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Product promise

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