Anti-CCDC6 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCDC6 antibody (AB235912)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for CCDC6 using ab235912 at 1/166 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC6 antibody (AB235912)

Paraffin-embedded human small intestine tissue stained for CCDC6 with ab235912 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

Supplier Data

Western blot - Anti-CCDC6 antibody (AB235912)

All lanes:

Western blot - Anti-CCDC6 antibody (ab235912) at 1/500 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 53 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC6 antibody (AB235912)

Paraffin-embedded human prostate cancer tissue stained for CCDC6 with ab235912 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.