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Rabbit Polyclonal CCDC65 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (AB122482), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (AB122482), expandable thumbnail
  • Western blot - Anti-CCDC65 antibody (AB122482), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXS2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/250.00000 - 1/500.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-4.00000 µg/mL

Notes

Recommend PFA Fixation and Triton X-100 treatment

Target data

Function

Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes (By similarity). Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with DRC1 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules (PubMed:24094744).

Alternative names

Recommended products

Rabbit Polyclonal CCDC65 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXS2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CCDC65 also known as coiled-coil domain-containing protein 65 plays an essential role in the mechanical structure of cilia. It is a protein with a mass of approximately 58 kDa. CCDC65 is primarily found in ciliated tissues where it forms a critical part of the cilium's internal structure. The protein is associated with the axonemal organization necessary for ciliary beating and movement.

Biological function summary

This protein serves as an important component of ciliary function. CCDC65 is a part of the nexin-dynein regulatory complex which stabilizes microtubule doublets in cilia and flagella. This stabilization ensures proper bending and motility of cilia which is pivotal for cellular processes such as fluid movement and signal transduction. CCDC65 interacts with other ciliary proteins facilitating coordinated bending essential for effective ciliary movement.

Pathways

Several cellular mechanisms involve CCDC65 in the processes linked to the assembly and functioning of motile cilia. It plays an important role in the ciliary beat regulation pathway closely interacting with proteins such as GAS8 and DRC3 to maintain effective ciliary motion. These relationships enable CCDC65 to participate actively in the pathways governing cilia biogenesis and the regulation of ciliary activity.

Associated diseases and disorders

Defects in CCDC65 are connected to primary ciliary dyskinesia (PCD) a condition characterized by impaired ciliary function leading to chronic respiratory tract infections and other respiratory issues. Research has also pointed to its association with situs inversus due to its role in ciliary motion necessary for determining left-right body asymmetry. Mutations in CCDC65 and its interaction partners GAS8 and DRC3 play a critical role in these pathological conditions highlighting its relevance in understanding genetic disorders involving ciliary dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (ab122482), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (ab122482)

    Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122482 1-4 µg/ml. Cells treated with PFA/Triton X-100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (ab122482), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (ab122482)

    ab122482 at 1/200 dilution staining CCDC65 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

  • Western blot - Anti-CCDC65 antibody (ab122482), expandable thumbnail

    Western blot - Anti-CCDC65 antibody (ab122482)

    All lanes: Western blot - Anti-CCDC65 antibody (ab122482) at 1/250 dilution

    Lane 1: RT-4 cell lysate

    Lane 2: U-251 MG cell lysate

    Lane 3: Human plasma lysate

    Lane 4: Human liver tissue lysate

    Lane 5: Human tonsil tissue lysate

    Developed using the ECL technique.

    Predicted band size: 57 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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