AB122482

Anti-CCDC65 antibody

Name: Anti-CCDC65 antibody (ab122482) | Abcam
Brand: Abcam Limited
SKU: AB122482

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(2 Publications)

Rabbit Polyclonal CCDC65 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus.

View Alternative Names

CCDC65, Dynein regulatory complex subunit 2, Coiled-coil domain-containing protein 65, Testis development protein NYD-SP28

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (AB122482)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (AB122482)

ab122482 at 1/200 dilution staining CCDC65 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (AB122482)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (AB122482)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122482 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Unknown

Western blot - Anti-CCDC65 antibody (AB122482)

All lanes:

Western blot - Anti-CCDC65 antibody (ab122482) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 57 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CCDC65 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IXS2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "Recommend PFA Fixation and Triton X-100 treatment" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCDC65 also known as coiled-coil domain-containing protein 65 plays an essential role in the mechanical structure of cilia. It is a protein with a mass of approximately 58 kDa. CCDC65 is primarily found in ciliated tissues where it forms a critical part of the cilium's internal structure. The protein is associated with the axonemal organization necessary for ciliary beating and movement.

Biological function summary

This protein serves as an important component of ciliary function. CCDC65 is a part of the nexin-dynein regulatory complex which stabilizes microtubule doublets in cilia and flagella. This stabilization ensures proper bending and motility of cilia which is pivotal for cellular processes such as fluid movement and signal transduction. CCDC65 interacts with other ciliary proteins facilitating coordinated bending essential for effective ciliary movement.

Pathways

Several cellular mechanisms involve CCDC65 in the processes linked to the assembly and functioning of motile cilia. It plays an important role in the ciliary beat regulation pathway closely interacting with proteins such as GAS8 and DRC3 to maintain effective ciliary motion. These relationships enable CCDC65 to participate actively in the pathways governing cilia biogenesis and the regulation of ciliary activity.

Defects in CCDC65 are connected to primary ciliary dyskinesia (PCD) a condition characterized by impaired ciliary function leading to chronic respiratory tract infections and other respiratory issues. Research has also pointed to its association with situs inversus due to its role in ciliary motion necessary for determining left-right body asymmetry. Mutations in CCDC65 and its interaction partners GAS8 and DRC3 play a critical role in these pathological conditions highlighting its relevance in understanding genetic disorders involving ciliary dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes (By similarity). Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with DRC1 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules (PubMed : 24094744).

See full target information CCDC65

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:154 PubMed40500776

2025

OTUD1 exacerbates sepsis-associated encephalopathy by promoting HK2 mitochondrial release to drive microglia pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Jing,Hailong Gong,Han Wang,Jing Zuo,Die Wu,Huifan Liu,Xing Wang,Min Yuan,Yun Xia,Tongtong Du,Wanhong Liu,Xiaojing Wu,Xuemin Song

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 32:121-132 PubMed38008820

2023

Ropivacaine suppresses the progression of renal cell carcinoma through regulating the lncRNA RMRP/EZH2/CCDC65 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfen Xiong,Xiaolan Zheng,Huangying Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCDC65 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (AB122482)

Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (AB122482)

Western blot - Anti-CCDC65 antibody (AB122482)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise