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AB222969

Anti-CCDC8 antibody

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Rabbit Polyclonal CCDC8 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 8 aa 200 to C-terminus.

View Alternative Names

Coiled-coil domain-containing protein 8, CCDC8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC8 antibody (AB222969)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCDC8 antibody (AB222969)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CCDC8 (green) using ab222969 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Western blot - Anti-CCDC8 antibody (AB222969)
  • WB

Supplier Data

Western blot - Anti-CCDC8 antibody (AB222969)

All lanes:

Western blot - Anti-CCDC8 antibody (ab222969) at 1/2000 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 59 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 8 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H0W5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CCDC8 protein known as Coiled-Coil Domain Containing 8 has a molecular mass of approximately 64 kDa. This protein is primarily expressed in the brain and other tissues where it plays a part in various cellular functions. CCDC8 is involved in processes that require structural support reflecting its high presence in complex cellular environments. Alternate names for CCDC8 include 3M2 or C3orf10 which sometimes appear in research literature.
Biological function summary

CCDC8 interacts in the cell's cytoskeleton organization. This protein is part of the 3M complex which includes proteins such as CUL7 and OBSL1. The 3M complex is involved in maintaining cellular integrity and influencing cell cycle regulation. CCDC8 helps in achieving appropriate responses to cellular stress conditions ensuring proper cell function and survival.

Pathways

You can find CCDC8 active in the DNA damage response and cell cycle pathways. These pathways are critical for maintaining genomic stability and CCDC8 has related interactions with proteins like TP53 a well-known tumor suppressor. CCDC8 also interacts in growth regulatory pathways linking it with other influence sensors that control cell division and differentiation.

CCDC8 mutations relate to 3M syndrome a disorder characterized by growth retardation. This syndrome links CCDC8 with proteins CUL7 and OBSL1 highlighting their combined role in skeletal development. Additionally CCDC8 has associations with cancer as its interaction with TP53 influences tumor growth regulation. Understanding these links aids studies in genetic disorders and cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed : 24793695, PubMed : 24793696). Required for localization of CUL7 to the centrosome (PubMed : 24793695).
See full target information Coiled-coil domain-containing protein 8
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Product promise

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